机构：[1]College of Basic courses, Guangdong Pharmaceutical University, Guangzhou 510006, China[2]Division of Education and Research, Shantou Central Hospital, Shantou 515031, China,College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China[3]College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China[4]Institute of Geriatrics, Henan College of Traditional Chinese Medicine, Zhengzhou 450003, China
To examine the neuroprotective effect of extract from Naomaitong following focal cerebral ischemia reperfusion induced by occlusion of middle cerebral artery (MCA), and to determine the biochemical alterations in urine using proton nuclear magnetic resonance spectroscopy and principal component analysis.
Wistar rats were randomly assigned to three groups: sham-operated group, MCA focal cerebral ischemia reperfusion model group, and active extract of Naomaitong treatment group. The model was established by an improved MCA occlusion (MCAO) method. Sham-operated rats received the same surgical procedure, but without occlusion. The Naomaitong treatment group were treated with active extract from Naomaitong at a dose of 3.0 gkg-1d-1. Brain tissues and urine samples were collected from all groups for histopathological assessment and proton nuclear magnetic resonance spectroscopy-based metabonomics, respectively.
Hematoxylin-eosin and triphenyl tetrazolium chloride staining of brain tissues showed a significant decrease in cerebral infarction area in the Naomaitong group. In model rats, metabonomic analyses showed increased urinary levels of glutamate, taurine, trimetlylamine oxide, betaine, and glycine, and reduced levels of creatinine and creatine. Naomaitong regulated the metabolic changes by acting on multiple metabolic pathways, including glycine metabolism, glutaminolysis, transmethylation metabolism and creatinine metabolism.
These data demonstrate that extract from Naomaitong is neuroprotective against focal cerebral ischemia induced by MCAO, and can alleviate biochemical changes in urinary metabolism. Metabonomics may be a useful approach for assessing the biochemical mechanisms underlying the neuroprotective actions of extract from Naomaitong.
基金：
Supported by National Natural Science Foundation of China
(Study on the Material Basis and the Ratio of the Effective
Components of Naodesheng Based on the Combination of
Fingerprint and Metabolic Network, No. 81274059; Study on
the Material Basis of Naomaitong in the Treatment of Ischemic
Stroke Based on the in vivo Dynamic Effect and Bioinformatics,
No. 81274060; Study on the in vivo Process and
Compatibility Rule of Naomaitong Based on the PK-PD of Effective
Components and the Multiobjective Optimization,
No. 81473413)
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|4 区医学
小类|4 区全科医学与补充医学
最新[2025]版：
大类|4 区医学
小类|4 区全科医学与补充医学
第一作者：
第一作者机构：[1]College of Basic courses, Guangdong Pharmaceutical University, Guangzhou 510006, China
通讯作者：
通讯机构：[4]Institute of Geriatrics, Henan College of Traditional Chinese Medicine, Zhengzhou 450003, China[*1]College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
推荐引用方式(GB/T 7714)：
Yang Yongxia,Chen Xi,Wang Shumei,et al.Neuroprotective effect of Naomaitong extract following focal cerebral ischemia induced by middle cerebral artery occlusion in rats.[J].Journal of Traditional Chinese Medicine.2017,37(3):333-340.
APA：
Yang Yongxia,Chen Xi,Wang Shumei,Wang Zhanhong,Li Jiansheng&Liang Shengwang.(2017).Neuroprotective effect of Naomaitong extract following focal cerebral ischemia induced by middle cerebral artery occlusion in rats..Journal of Traditional Chinese Medicine,37,(3)
MLA：
Yang Yongxia,et al."Neuroprotective effect of Naomaitong extract following focal cerebral ischemia induced by middle cerebral artery occlusion in rats.".Journal of Traditional Chinese Medicine 37..3(2017):333-340
