A novelly synthesized phenanthroline derivative is a promising DNA-damaging anticancer agent inhibiting G1/S checkpoint transition and inducing cell apoptosis in cancer cells.
The study mainly aimed to determine the biological function of a novelly synthesized phenanthroimidazole derivative, named L233, and to explore its potential mechanisms.
Cell survival was examined using the MTT assays, and the DNA-damaging role of L233 was explored using the comet assay. Moreover, the western blotting assays and immunofluorescence assays were used to detect DNA damage biomarkers. Afterward, the flow cytometry was used to assess the effects of L233 on cell cycle distribution. As for the detection of cell apoptosis upon L233 treatment, the Hoechst 33342 staining, flow cytometry, and western blotting assays were all put into practice.
We find that L233 inhibits tumor cell growth more efficiently and safely than cisplatin. Moreover, it is a DNA-damaging agent, interrupting the cell cycle G1/S checkpoint transition and inducing cell apoptosis by not only activating ATM/CHK1 signaling pathway, but also targeting CHK1 to reduce the expression of RAP80 and PARP-1 to compromise the DNA damage repair in tumor cells.
In summary, L233 is a promising anticancer drug for the development of novel chemotherapies in the future.
基金：
This study is funded by Shanghai Young
Science and Technology Talents Sailing Program (Grant No.
15YF1409300) and Natural Science Foundation of China (Grant No.
81272292, 81071524, 81171778, 81371913, 81472624, 31171086,
and 81472124).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|3 区医学
小类|3 区药学4 区肿瘤学
最新[2025]版：
大类|4 区医学
小类|4 区肿瘤学4 区药学
第一作者：
第一作者机构：[1]Department of Clinical Laboratory Medicine, Shanghai Tenth People’s Hospital of Tongji University, Shanghai 200072, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Ni Zhen,Qingyuan Yang,Qiong Wu,et al.A novelly synthesized phenanthroline derivative is a promising DNA-damaging anticancer agent inhibiting G1/S checkpoint transition and inducing cell apoptosis in cancer cells.[J].Cancer chemotherapy and pharmacology.2016,77(1):169-80.doi:10.1007/s00280-015-2894-5.
APA：
Ni Zhen,Qingyuan Yang,Qiong Wu,Xinyi Zhu,Yue Wang...&Yongchun Yu.(2016).A novelly synthesized phenanthroline derivative is a promising DNA-damaging anticancer agent inhibiting G1/S checkpoint transition and inducing cell apoptosis in cancer cells..Cancer chemotherapy and pharmacology,77,(1)
MLA：
Ni Zhen,et al."A novelly synthesized phenanthroline derivative is a promising DNA-damaging anticancer agent inhibiting G1/S checkpoint transition and inducing cell apoptosis in cancer cells.".Cancer chemotherapy and pharmacology 77..1(2016):169-80
