机构：[1]Department of Pharmacology, Guangdong MedicalUniversity, 523808 Dongguan, China[2]Department of Orthopaedics and Traumatology, The ChineseUniversity of Hong Kong, Prince of Wales Hospital, Shatin,Hong Kong, China[3]Affiliated Hospital of Guangdong Medical University,524023 Zhanjiang, China[4]School of Nursing, Guangdong Medical University,523808 Dongguan, China[5]Institution of Traditional Chinese Medicine and NewPharmacy Development, Guangdong Medical University,523808 Dongguan, China[6]Guangdong Key Laboratory for Research and Developmentof Natural Drugs, Guangdong Medical University,524023 Zhanjiang, China
Amentoflavone is a bioflavonoid found in a variety of traditional Chinese medicines such as Gingko and Selaginella tamariscina. It has been reported that amentoflavone has anti-inflammatory, antioxidant, antiviral and anticancer effects. However, the effect of amentoflavone on osteogenic differentiation of human mesenchymal stem cells (hMSCs) has not been studied. In this study, we aim to explore the effect of amentoflavone on the proliferation and osteogenic differentiation of hMSCs. The results showed that amentoflavone significantly enhanced the proliferation, alkaline phosphatase (ALP) activity and mineralization in hMSCs. Western blot analysis revealed that the expression of runt-related transcription factor 2 and osterix proteins was upregulated in amentoflavone-treated hMSCs. Furthermore, we investigated the possible signaling pathways responsible for osteogenic differentiation of hMSCs by amentoflavone. We found that amentoflavone significantly increased the levels of phosphorylated JNK and p-p38. The amentoflavone-induced increases of ALP and mineralization were significantly diminished when the JNK and p38 MAPK pathways were blocked by selected inhibitors (SP600125, SB203580) in hMSCs. Furthermore, in vivo evidence indicated that amentoflavone protected against the dexamethasone-induced inhibition of osteoblast differentiation in tg(sp7:egfp) zebrafish larvae. Thus, we showed for the first time that amentoflavone improves the osteogenesis of hMSCs through the JNK and p38 MAPK pathway. Amentoflavone may be beneficial in treating bone-related disorders.
基金：
This project was supported by the National
Natural Science Foundation of China (30772768, 81102450), Science
and Technology Planning Project of Guangdong Province
(2013B031800013), Natural Science Foundation of Guangdong Province
(2014A030313534), Administration of Traditional Chinese
Medicine of Guangdong Province (20151263), Social Science and
Technology Development Project of Dongguan (2014108101052),
Science and Technology Planning Project for Medical Treatment and
Public Health of Dongguan (2014105101294) and Initiating Fund of
Scientific Research for Doctors of Guangdong Medical University
(B2013016).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|4 区医学
小类|4 区药物化学4 区药学
最新[2025]版：
大类|4 区医学
小类|4 区药物化学4 区药学
第一作者：
第一作者机构：[1]Department of Pharmacology, Guangdong MedicalUniversity, 523808 Dongguan, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Pharmacology, Guangdong MedicalUniversity, 523808 Dongguan, China[5]Institution of Traditional Chinese Medicine and NewPharmacy Development, Guangdong Medical University,523808 Dongguan, China[6]Guangdong Key Laboratory for Research and Developmentof Natural Drugs, Guangdong Medical University,524023 Zhanjiang, China
推荐引用方式(GB/T 7714)：
Zha Xuan,Xu Zhoumei,Liu Yuyu,et al.Amentoflavone enhances osteogenesis of human mesenchymal stem cells through JNK and p38 MAPK pathways.[J].Journal of natural medicines.2016,70(3):634-44.doi:10.1007/s11418-016-0993-1.
APA：
Zha Xuan,Xu Zhoumei,Liu Yuyu,Xu Liangliang,Huang Hongxin...&Xu Daohua.(2016).Amentoflavone enhances osteogenesis of human mesenchymal stem cells through JNK and p38 MAPK pathways..Journal of natural medicines,70,(3)
MLA：
Zha Xuan,et al."Amentoflavone enhances osteogenesis of human mesenchymal stem cells through JNK and p38 MAPK pathways.".Journal of natural medicines 70..3(2016):634-44
