机构：[1]The Clinical laboratory, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China[2]The Third Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China
Circular RNAs (circRNAs) are emerging as important regulators in bone metabolism, which is mediated by microRNA (miRNA) sponges. However, it is not clear how circRNA regulates osteogenic differentiation of human bone marrow mesenchymal stem cells (hBM-MSCs).Therefore, based on the previous circRNA chip results, hsa_circ_0006766, which is differentially expressed in the osteogenic differentiation of hBM-MSCs, was screened out, and bioinformatics analysis was performed to predict potential target miRNAs. During osteogenic differentiation of hBM-MSCs, hsa_circ_0006766 and its target miRNAs (miR-4739, miR-619-5p, miR-5787, miR-7851-3p, and miR-3192-5p) were detected by quantitative Real Time-PCR (qRT-PCR). Target gene prediction for the differentially expressed target miRNAs was performed, and target genes were validated by dual-luciferase reporter gene assay and qRT-PCR. It is shown that hsa_circ_0006766 was up-regulated and miR-4739 was down-regulated during osteogenic differentiation of hBM-MSCs.Moreover, the target gene Notch2 was predicted to be highly expressed during osteogenic differentiation. And dual-luciferase assay proved that Notch2 was the gene targeting to miR-4739. Taken together, our finding confirmed that hsa_circ_0006766 may act as a major regulatory part in osteogenic differentiation of hBM-MSCs via an hsa_circ_0006766-miR-4739-Notch2 regulatory axis. Accordingly, hsa_circ_0006766 may affect the development of osteoporosis and may thus become a therapeutic target.
基金：
National Natural Science Foundation of China [81973633], Key Laboratory Construction Project of Guangzhou Science and Technology Bureau[202102100007], Collaborative innovation team project of double first-class and high-level universities in Guangzhou University of traditional Chinese Medicine[2021xk62] and Key research projects of first-class disciplines of Guangzhou University of Chinese medicine in 2020[reference number: Guangzhou University of Chinese medicine2020Reference No. 62] ;Key research projects of first-class disciplines of Guangzhou University of Chinese medicine in 2020 [Reference No. 62];the National Natural Science Foundation of China [81973633];
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|4 区生物
小类|4 区生物工程与应用微生物
最新[2025]版：
大类|4 区生物学
小类|4 区生物工程与应用微生物
第一作者：
第一作者机构：[1]The Clinical laboratory, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China[2]The Third Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China
共同第一作者：
通讯作者：
通讯机构：[1]The Clinical laboratory, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China[2]The Third Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China[*1]The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, 261 Longxi Avenue, Liwan District, Guangzhou, Guangdong, China
推荐引用方式(GB/T 7714)：
Guo Zhaodi,Xie Manlin,Zou Yanfang,et al.Circular RNA Hsa_circ_0006766 targets microRNA miR-4739 to regulate osteogenic differentiation of human bone marrow mesenchymal stem cells.[J].Bioengineered.2021,12(1):5679-5687.doi:10.1080/21655979.2021.1967712.
APA：
Guo Zhaodi,Xie Manlin,Zou Yanfang,Liang Qianxin,Liu Fubin...&Zhao Kewei.(2021).Circular RNA Hsa_circ_0006766 targets microRNA miR-4739 to regulate osteogenic differentiation of human bone marrow mesenchymal stem cells..Bioengineered,12,(1)
MLA：
Guo Zhaodi,et al."Circular RNA Hsa_circ_0006766 targets microRNA miR-4739 to regulate osteogenic differentiation of human bone marrow mesenchymal stem cells.".Bioengineered 12..1(2021):5679-5687
