机构：[1]Department of Molecular Biology, School of Traditional Chinese Medicine[2]Department of Radiotherapy, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
Curcumin (Cur) exhibits radiosensitization effects to a variety of malignant tumors. The present study investigates the radiosensitizing effect of Cur on nasopharyngeal carcinoma (NPC) cells and whether its mechanism is associated with microRNA-593 (miR-593) and multidrug resistance gene 1 (MDR1). A clonogenic assay was performed to measure the radiosensitizing effect. The expression of miR-593 and MDR1 was analyzed by quantitative polymerase chain reaction (qPCR) or western blot assay. A transplanted tumor model was established to identify the radiosensitizing effect in vivo. A luciferase-based reporter was constructed to evaluate the effect of direct binding of miR-593 to the putative target site on the 3' UTR of MDR1. The clonogenic assay showed that Cur enhanced the radiosensitivity of cells. Cur (100 mg/kg) combined with 4 Gy irradiation inhibited the growth of a transplanted tumor model in vivo, resulting in the higher inhibition ratio compared with the radiotherapy-alone group. These results demonstrated that Cur had a radiosensitizing effect on NPC cells in vivo and in vitro; Cur-mediated upregulation of miR-593 resulted in reduced MDR1 expression, which may promote radiosensitivity of NPC cells.
基金：
The present study was supported by the National Natural Science Foundation of China (grant nos. 81173616, 81202430 and 81302948) and Guangdong Science and Technology Project (grant no. 2013A032500003), and Science and Technology Project of Haizhu District (grant no. 2013‑cg‑29); the authors thank medical personnel Mr. Jiabin Liu and Ms. Huarui Niu of NanFang Hospital for providing X‑ray radiation equipment.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|4 区医学
小类|4 区肿瘤学
最新[2025]版：
大类|4 区医学
小类|4 区肿瘤学
第一作者：
第一作者机构：[1]Department of Molecular Biology, School of Traditional Chinese Medicine
共同第一作者：
通讯作者：
通讯机构：[1]Department of Molecular Biology, School of Traditional Chinese Medicine[*1]Department of Molecular Biology, School of Traditional Chinese Medicine, Southern Medical University, 1,838 Guangzhou Avenue North, Guangzhou, Guangdong 510515, P.R. China
推荐引用方式(GB/T 7714)：
Fan Haoning,Shao Meng,Huang Shaohui,et al.MiR-593 mediates curcumin-induced radiosensitization of nasopharyngeal carcinoma cells via MDR1.[J].Oncology letters.2016,11(6):3729-3734.doi:10.3892/ol.2016.4438.
APA：
Fan Haoning,Shao Meng,Huang Shaohui,Liu Ying,Liu Jie...&Fan Qin.(2016).MiR-593 mediates curcumin-induced radiosensitization of nasopharyngeal carcinoma cells via MDR1..Oncology letters,11,(6)
MLA：
Fan Haoning,et al."MiR-593 mediates curcumin-induced radiosensitization of nasopharyngeal carcinoma cells via MDR1.".Oncology letters 11..6(2016):3729-3734
