The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway.
机构：[1]Key Laboratory of Hunan Province for Traditional Chinese Medicine in Obstetrics and Gynecology Research, Hunan Provincial Maternal and Child Health Care Hospital, No. 53 XiangChun Road, Changsha 410008, China.[2]School of Pharmaceutical Sciences, Central South University, Changsha 410011, China.[3]College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410007, China.[4]Clinic Pharmacy Research Laboratory, the Second Xiangya Hospital of Central South University, Changsha 410011, China.[5]Guangzhou Institute of Advanced Technology, Chinese Academy of Sciences, No. 1121 Haibin Road, Guangzhou 511458, Guangdong, China.
Andrographis paniculata (Burm. f.) Nees (APN), a principal constituent of a famous traditional Chinese medicine Fukeqianjin tablet which is used for the treatment of pelvic inflammatory disease (PID), has been reported to have anti-inflammatory effect in vitro. However, whether it has pharmacological effect on PID in vivo is unclear. Therefore, the aim of this study is to test the anti-inflammatory effect of APN and illuminate a potential mechanism.
Thirty-six female specific pathogen-free SD rats were randomly divided into control group, PID group, APN1 group, APN2 group, APN3 group and prednisone group. Pathogen-induced PID rats were constructed. The APN1, APN2 and APN3 group rats were orally administrated with APN extract at different levels. The prednisone group rats were administrated with prednisone. Eight days after the first infection, the histological examination of upper genital tract was carried out, and enzyme-linked immunosorbent assay (ELISA) was carried out using homogenate of the uterus and fallopian tube. Furthermore, immunohistochemical evaluations of NF-κB p65 and IκB-α in uterus was conducted.
APN obviously suppressed the infiltrations of neutrophils and lymphocytes, and it could significantly reduce the excessive production of cytokines and chemokines including IL-1β, IL-6, CXCL-1, MCP-1 and RANTES in a dose-dependent manner. Furthermore, APN could block the pathogen-induced activation of NF-κB pathway.
APN showed potent anti-inflammatory effect on pathogen-induced PID in rats, with a potential mechanism of inhibiting the NF-κB signal pathway.
基金：
This research was supported by National Natural Science Foundation
(No.81501218), China Postdoctoral Science Foundation (No. 2015 M582330),
and Science and Technology Project of Hunan Province (No. 2015RS4054).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|4 区医学
小类|3 区全科医学与补充医学
最新[2025]版：
无
第一作者：
第一作者机构：[1]Key Laboratory of Hunan Province for Traditional Chinese Medicine in Obstetrics and Gynecology Research, Hunan Provincial Maternal and Child Health Care Hospital, No. 53 XiangChun Road, Changsha 410008, China.[2]School of Pharmaceutical Sciences, Central South University, Changsha 410011, China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Wei Zou,Zuoqi Xiao,Xiaoke Wen,et al.The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway.[J].BMC complementary and alternative medicine.2016,16(1):483.doi:10.1186/s12906-016-1466-5.
APA：
Wei Zou,Zuoqi Xiao,Xiaoke Wen,Jieying Luo,Shuqiong Chen...&Jingyu He.(2016).The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway..BMC complementary and alternative medicine,16,(1)
MLA：
Wei Zou,et al."The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway.".BMC complementary and alternative medicine 16..1(2016):483
