机构：[1]Department of Pathophysiology, Key Laboratory of State Administration of Traditional Chinese Medicine of the People’s Republic of China, School of Medicine, Jinan University, No. 601 Huangpu Avenue West, 510632 Guangzhou, Guangdong, China[2]Department of Pathology, Municipal People’s Hospital, 243000 Maanshan, Anhui, China[3]Department of Gynecology, Clifford Hospital, 511495 Guangzhou, Guangdong, China[4]Texas A & M University, College Station, TX 77843, USA
Neuronal apoptosis is an important event in hypoxia/reoxygenation (H/R)-induced neuronal injury. Senegenin (Sen), the predominant and most active component in Radix Polygalae root extracts, displays anti-apoptotic and anti-oxidative properties. Sen protects against H/R-induced neuronal apoptosis of highly differentiated PC12 cells and primary cortical neurons. Sen has also been investigated as a source of potential therapeutic targets. In this study, a proteomic approach was used to identify Sen-regulated proteins in PC12 cells. We found that Sen protected against H/R-induced neuronal apoptosis by upregulating RhoGDIα protein expression. The regulatory functions of RhoGDIα were investigated by knocking down RhoGDIα expression in PC12 cells using small interfering RNA (siRNA), followed by quantification of apoptosis and then altering the expression levels of apoptosis-related proteins. Our data show that after silencing RhoGDIα, the neuroprotective effects of Sen on H/R-induced PC12 cell apoptosis were absent. Furthermore, RhoGDIα silencing alleviated the Sen-mediated inhibition of the JNK pathway. Therefore, these findings indicated that Sen attenuates H/R-induced neuronal apoptosis by upregulating RhoGDIα expression and inhibiting the JNK pathway. In addition to the mechanism underlying neuroprotective effects of Sen, RhoGDIα was identified as a putative target of Sen based on a primary rat cortical neuron model of H/R-induced injury.
基金：
National Program
on Key Basic Research Project (973 Program, 2011CB707501), the
Project of Science and Technology of Guangzhou (2014J4100098), and
the Fundamental Research Funds for the Central Universities in China
(No. 21613401 and 21612446).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2014]版：
大类|2 区医学
小类|2 区神经科学
最新[2025]版：
大类|2 区医学
小类|2 区神经科学
第一作者：
第一作者机构：[1]Department of Pathophysiology, Key Laboratory of State Administration of Traditional Chinese Medicine of the People’s Republic of China, School of Medicine, Jinan University, No. 601 Huangpu Avenue West, 510632 Guangzhou, Guangdong, China[2]Department of Pathology, Municipal People’s Hospital, 243000 Maanshan, Anhui, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Li Xuemin,Zhao Yandong,Liu Panhong,et al.Senegenin Inhibits Hypoxia/Reoxygenation-Induced Neuronal Apoptosis by Upregulating RhoGDIα.[J].Molecular neurobiology.2015,52(3):1561-1571.doi:10.1007/s12035-014-8948-6.
APA：
Li Xuemin,Zhao Yandong,Liu Panhong,Zhu Xiaoqing,Chen Minyi...&Qi Renbin.(2015).Senegenin Inhibits Hypoxia/Reoxygenation-Induced Neuronal Apoptosis by Upregulating RhoGDIα..Molecular neurobiology,52,(3)
MLA：
Li Xuemin,et al."Senegenin Inhibits Hypoxia/Reoxygenation-Induced Neuronal Apoptosis by Upregulating RhoGDIα.".Molecular neurobiology 52..3(2015):1561-1571
