机构：[1]Teaching Hospital of Chengdu University of TCM, Chengdu 610072, Sichuan, China.[2]Department of Dermatology, Affiliated Hospital of Guangdong Medical College, No.57 Peoples Avenue South, Zhanjiang 524001, Guangdong, China[3]Laboratory Animal Center, Anhui Medical University, Hefei 230032, Anhui, China.[4]Department of Dermatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China.[5]Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei 230032, Anhui, China[6]Department of Dermatology, The Second Affiliated Hospital, Anhui Medical University Hefei, 678 Furong Road, Hefei 230601, Anhui, China.
Systemic lupus erythematosus (SLE) is a prototypic systemic autoimmune disease. Complement component 4 (C4) has be proved to play a role in pathogenesis of SLE. In the present study, we investigated the effect of C4 on T cells differentiation.
Thirty SLE patients were included in this study. CD4+ T cells were isolated from healthy subjects, and dendritic cells (DCs) were isolated from healthy subjects or SLE patients. C4 was supplemented to co-incubate with T cells and DCs.
Serum C4 concentration was positively correlated with regulatory T cell (Treg) percentage (R(2) = 0.5907, p < 0.001) and TGFβ concentration (R(2) = 0.5641, p < 0.001) in SLE patients. Different concentrations of C4 had no effect on T cells differentiation. Co-incubated T cells with DCs and C4 for 7 days, the Treg percentage and TGF-β concentration were significantly elevated. In addition, pre-treated DCs (from healthy subjects or SLE patients) with C4 and then co-incubated with T cells, the increases of Treg percentage and TGF-β concentration were also observed.
C4 takes part in T cells differentiation to Treg cells via DCs.
基金：
National Natural Science Foundation of China
Grant 81201226 and 81472880.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2014]版：
大类|3 区生物
小类|3 区生化与分子生物学
最新[2025]版：
大类|2 区生物学
小类|2 区生化与分子生物学
第一作者：
第一作者机构：[1]Teaching Hospital of Chengdu University of TCM, Chengdu 610072, Sichuan, China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Cheng Hong-Bin,Chen Rong-Yi,Wu Jing-Ping,et al.Complement C4 induces regulatory T cells differentiation through dendritic cell in systemic lupus erythematosus.[J].Cell & bioscience.2015,5:73.doi:10.1186/s13578-015-0052-8.
APA：
Cheng Hong-Bin,Chen Rong-Yi,Wu Jing-Ping,Chen Li,Liang Yan-Hua...&Shi Jian-Qiang.(2015).Complement C4 induces regulatory T cells differentiation through dendritic cell in systemic lupus erythematosus..Cell & bioscience,5,
MLA：
Cheng Hong-Bin,et al."Complement C4 induces regulatory T cells differentiation through dendritic cell in systemic lupus erythematosus.".Cell & bioscience 5.(2015):73
生物学