Electroacupuncture treatment contributes to the downregulation of neuronal nitric oxide synthase and motoneuron death in injured spinal cords following root avulsion of the brachial plexus.
机构：[1]Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080[2]Encephalopathy Center, Guangdong Hospital of Traditional Chinese Medicine, Guangzhou, Guangdong 510120, P.R. China广东省中医院
This study was performed in order to investigate the effect of electroacupuncture (EA) on motoneurons and the expression of neuronal nitric oxide synthase (nNOS) following brachial plexus root avulsion (BPRA). A total of 40 female Sprague-Dawley rats underwent BPRA (5th cervical-1st thoracic) and were randomly divided into the avulsion plus EA stimulation (AV+EA) and AV groups. The AV+EA group received a continuous 20-Hz asymmetric bidirectional disperse-dense wave at the acupuncture points (acupoints) of Dazhui (DU4) and Shousanli (LI10) for 15 min on alternate days until the animals were sacrificed, at 1, 2, 3 and 6 weeks. The AV group received no treatment. The cryostat sections of the 7th cervical segments were prepared and stained with neuronal nitric oxide synthase nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) and histochemically stained and counterstained with neutral red (NR). The number of nNOS-positive motoneurons on the lesion side and survived motoneurons on both sides of the 7th cervical segments were blindly counted and compared between the two groups. The results demonstrated that the number of nNOS-positive motoneurons was significantly lower in the AV+EA group compared with that in the AV group and the percentage of survived motoneurons was significantly higher compared with that of the AV group at 2 and 3 weeks. However, the number of nNOS-positive motoneurons and the percentage of survived motoneurons were not significantly different between the two groups at 1 and 6 weeks. These results indicated that, during the early period after BPRA, EA stimulation at the acupoints of Dazhui (DU4) and Shousanli (LI10) may significantly reduce the number of nNOS-positive motoneurons and protect against motoneuron death.
基金：
National Natural Science Foundation of China (nos. 81070955, 31171290 and
81303115), the Natural Science Foundation of Guangdong
Province (nos. S20134203030 and S2013040016915) and the
Postdoctoral Foundation of China (no. BBK42913K09).
基金编号：nos. 81070955, 31171290 and81303115
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2013]版：
无
最新[2025]版：
大类|4 区医学
小类|4 区医学：研究与实验
第一作者：
第一作者机构：[1]Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080
通讯作者：
通讯机构：[1]Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080[2]Encephalopathy Center, Guangdong Hospital of Traditional Chinese Medicine, Guangzhou, Guangdong 510120, P.R. China[*1]Department of Anatomy, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan Er Road, Guangzhou, Guangdong 510080, P.R. China[*2]Enchephalopathy Center, Guangdong Hospital of Traditional Chinese Medicine, 111 Dade Road, Guangzhou, Guangdong 510120, P.R. China
推荐引用方式(GB/T 7714)：
Luo Haoxuan,Cheng Xiao,Tang Ying,et al.Electroacupuncture treatment contributes to the downregulation of neuronal nitric oxide synthase and motoneuron death in injured spinal cords following root avulsion of the brachial plexus.[J].Biomedical reports.2014,2(2):207-212.doi:10.3892/br.2013.212.
APA：
Luo Haoxuan,Cheng Xiao,Tang Ying,Ling Zemin&Zhou Lihua.(2014).Electroacupuncture treatment contributes to the downregulation of neuronal nitric oxide synthase and motoneuron death in injured spinal cords following root avulsion of the brachial plexus..Biomedical reports,2,(2)
MLA：
Luo Haoxuan,et al."Electroacupuncture treatment contributes to the downregulation of neuronal nitric oxide synthase and motoneuron death in injured spinal cords following root avulsion of the brachial plexus.".Biomedical reports 2..2(2014):207-212
