机构：[1]Department of Anesthesiology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, 510010, Guangdong Province, China[2]Department of Anesthesiology, Jiangxi Maternal and Child Health Hospital, Nanchang, 330000, Jiangxi Province, China[3]Department of Anesthesiology, Second Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou, 510120, Guangdong Province, China[4]Department of Anesthesiology, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, 07013, USA
We developed a cell penetrating peptide (CPP) Tat-LK15, as a siRNA carrier to target nNOS. The feasibility, stability, efficiency and selectivity of this peptide-siRNA complex were evaluated in rat neuronal cells. We also compared the new method with conventional siRNA carrier Lipofectamine (TM). It was found that the CPP Tat-LK15 effectively and specifically delivered nNOS-siRNA into Rat retinal ganglia (RGC-5) cells and silenced the expression of nNOS. The CPP Tat-LK15 can conjugate with siRNA to form stable complex at a ratio of 2:1 (peptide/siRNA, wow), which maintained stable in serum for as long as 4 h. The CPP Tat-LK15 was low-toxicity to cells, as the apoptosis rate of treat cells was not increased significantly when the used peptide lower than 10 mu g/mL. Moreover, the cellular uptake of nNOS siRNA by Rat Neurons-dorsal spinal cord (RNdsc) cells was also significantly more than naked siRNA by RNdsc cells. The CPP Tat-LK15 was an efficient and stable, and non-cytotoxic siRNA delivery to neurons and effectively silenced the nNOS expression. The CPP Tat-LK15 mediated siRNA delivery was a potential tool to treat neuropathic diseases involving NO or nNOS neurotoxic cascades. (C) 2017 Elsevier B.V. All rights reserved.
基金：
National Natural Science Foun-dation of China (81100817 and 81371233) and Science andTechnology Project of Guangdong Province (2014A020212555).
第一作者机构：[1]Department of Anesthesiology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, 510010, Guangdong Province, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Anesthesiology, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, 510010, Guangdong Province, China[*1]Department of Anesthesiology, Guangzhou GeneralHospital of Guangzhou Military Command, 111 Liuhua Road, Guangzhou, 510010,Guangdong Province, China.
推荐引用方式(GB/T 7714)：
Peng Jie,Rao Yun,Yang Xue,et al.Targeting neuronal nitric oxide synthase by a cell penetrating peptide Tat-LK15/siRNA bioconjugate[J].NEUROSCIENCE LETTERS.2017,650:153-160.doi:10.1016/j.neulet.2017.04.045.
APA：
Peng, Jie,Rao, Yun,Yang, Xue,Jia, Ji,Wu, Youping...&Tu, Weifeng.(2017).Targeting neuronal nitric oxide synthase by a cell penetrating peptide Tat-LK15/siRNA bioconjugate.NEUROSCIENCE LETTERS,650,
MLA：
Peng, Jie,et al."Targeting neuronal nitric oxide synthase by a cell penetrating peptide Tat-LK15/siRNA bioconjugate".NEUROSCIENCE LETTERS 650.(2017):153-160
