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A novel layer-by-layer self-assembly biomimetic hydroxyapatite-cell sheet for bone tissue engineering

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机构: [1]Department of Anatomy, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Guangdong Provincial Medical Biomechanical Key Laboratory, Southern Medical University, Guangzhou, 510515, China [2]Laboratory of Laser Medicine, Institute of Biomedical Engineering, Peking Union Medical College and Chinese Academy of Medical Sciences, Tianjin, 300192, China [3]Department of Traumatic Orthopedics, Ersha Island Branch Hospital, The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong Province
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关键词: Assembling layer by layer (LBL) Cell sheet technology (CST) Hydroxyapatite micro-particles (mHAP) Organic–Inorganic matrix

摘要:
In this paper, a new biological layer-by-layer (LBL) cell sheet was developed for bone tissue engineering based on the interaction between hydroxyapatite micro-particles (mHAP) and MC3T3-E1 osteoblast cells. The LBL film mimicked a natural combination of inorganic and organic matrix. The properties of the cell sheets were characterized by live/dead assay, hematoxylin-eosin (HE) staining and scanning electron microscope (SEM) observation. The resulting cell sheet had exhibited well cell viability with the integrity of the extracellular matrix and mHAP particles. The interface between cell sheet and hydrogel showed that the living cells in deep layer have the same morphological structure as the cells on the superficial layer. The cell sheet could apart from the cell culture dish through deposited mHAP across in the middle of cells and the dish. In addition, the cell sheet maintained a high osteogenic capacity and could be taken as a more ideal bio-system for the repair of damaged bones without PIPAAm or any other ancillary equipment. © 2016 American Scientific Publishers. All rights reserved.

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出版当年[2015]版:
大类 | 4 区 生物
小类 | 4 区 细胞与组织工程
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第一作者机构: [1]Department of Anatomy, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Guangdong Provincial Medical Biomechanical Key Laboratory, Southern Medical University, Guangzhou, 510515, China
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