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Administration of H2S improves erectile dysfunction by inhibiting phenotypic modulation of corpus cavernosum smooth muscle in bilateral cavernous nerve injury rats.

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收录情况： ◇ SCIE

作者：
Qinyu-Zeng[1] * ; Shuhua-He[1] * ; Fengzhi-Chen[2] * ; Li-Wang[1] ; Liren-Zhong[1] ; Jialiang-Hui[1] ; Wei-Ding[1,3] ; Junhong-Fan[1] ; Haibo-Zhang[1] ; Anyang-Wei[1] ;
机构： [1]Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. [2]Department of Urology, Shenzhen Second People's Hospital, the First Affiliated Hospital of Shenzhen University, Shenzhen, 518035, Guangdong, China. [3]Department of Urology, the First Affiliated Hospital of Guiyang University of Chinese Medicine, Guiyang, China
出处：
DOI： 10.1016/j.niox.2020.11.003
ISSN：

关键词： Bilateral cavernous nerve injury Erectile dysfunction Hydrogen sulfide Phenotype modulation Radical prostatectomy RhoA/ROCK

摘要：
Phenotypic modulation of Corpus Cavernosum Smooth Muscle Cells (CCSMCs) is an important step in the development and progression of bilateral cavernous nerve injury induced erectile dysfunction (BCNI-ED). To investigate the effect of exogenous hydrogen sulfide (H2S) on the phenotypic modulation of CCSMCs in BCNI-ED rats, a total of 18 male Sprague-Dawley rats were equally divided into 3 groups, including sham-operated (Sham) group, BCNI group and BCNI treated with NaHS (BCNI + NaHS) group. The treated group received intraperitoneal injection of NaHS (100 μmol kg-1day-1) for 4 weeks starting day 1 postoperatively. Erectile function was measured by the ratio of intracavernous pressure (ICP)/mean arterial pressure (MAP), and relevant tissues were harvested for Immunohistochemistry, Hematoxylin and eosin (H&E), Masson's trichrome staining, H2S fluorescent probe WSP-1 and Western blot. The primary CCSMCs were isolated and pretreatment with NaHS before exposed to PDGF-BB (platelet-derived growth factor). Relative expression mRNA and protein of phenotypic biomarkers, RhoA, ROCK-1 and cell cycle proteins were detected. Cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE), 3-mercaptopyruvate sulfurtransferase (3-MST) and H2S levels in penile tissue was significantly decreased in the BCNI group compared with the Sham group. Compared with the BCNI group, administration of NaHS significantly increased the ratio of ICP/MAP, ratio of smooth muscle to collagen, expressions of a-SMA, calponin and decreased the expression of OPN, collagen-I, RhoA, ROCK1 in the penile tissue. PDGF-BB-treated CCSMCs exhibited higher expression of OPN, RhoA, ROCK1, and lower α-SMA, calponin, which were attenuated by NaHS pretreatment. NaHS suppressed RhoA/ROCK activity and decreased the expression of CDK2, Cyclin E1, while increased the expression of P27kip1 induced by PDGF-BB in CCSMCs. Taken together, this study indicated that exogenous H2S inhibited the phenotypic modulation of CCSMCs by suppressing RhoA/ROCK1 signaling and affecting its downstream factor, CDK2, Cyclin E1, P27kip1, thereby improved BCNI rat erectile function. Copyright © 2020. Published by Elsevier Inc.

基金：
This study was supported by grants from the National Natural Science Foundation of China(81571433), the Natural Science Foundation of Guangdong Province, China (2017A030313453,2018A030313638, 2020A151501114), the Presidential Foundation of Nanfang Hospital, Southern Medical University (2017B001, 2017Z014), and Shenzhen Key Medical Discipline Construction Fund（No.SZXK020).
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外文
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中科院(CAS)分区：
出版当年[2020]版：
大类 | 2 区 生物
小类 | 3 区 生化与分子生物学 3 区 细胞生物学
最新[2025]版：
大类 | 3 区 生物学
小类 | 3 区 生化与分子生物学 4 区 细胞生物学
JCR分区：
出版当年[2019]版：
Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Q3 CELL BIOLOGY
最新[2023]版：
Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Q3 CELL BIOLOGY

影响因子： 最新[2023版] 最新五年平均 出版当年[2019版] 出版当年五年平均 出版前一年[2018版] 出版后一年[2020版]

第一作者：
第一作者机构： [1]Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Qinyu-Zeng,Shuhua-He,Fengzhi-Chen,et al.Administration of H2S improves erectile dysfunction by inhibiting phenotypic modulation of corpus cavernosum smooth muscle in bilateral cavernous nerve injury rats.[J].NITRIC OXIDE-BIOLOGY AND CHEMISTRY.2021,107:1-10.doi:10.1016/j.niox.2020.11.003.
APA：
Qinyu-Zeng,Shuhua-He,Fengzhi-Chen,Li-Wang,Liren-Zhong...&Anyang-Wei.(2021).Administration of H2S improves erectile dysfunction by inhibiting phenotypic modulation of corpus cavernosum smooth muscle in bilateral cavernous nerve injury rats..NITRIC OXIDE-BIOLOGY AND CHEMISTRY,107,
MLA：
Qinyu-Zeng,et al."Administration of H2S improves erectile dysfunction by inhibiting phenotypic modulation of corpus cavernosum smooth muscle in bilateral cavernous nerve injury rats.".NITRIC OXIDE-BIOLOGY AND CHEMISTRY 107.(2021):1-10

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