Characterization of chemical profile and quantification of major representative components of Wendan decoction, a classical traditional Chinese medicine formula
机构：[1]College of Pharmacy, Jinan University, Guangzhou, P.R. China[2]Department of Cardiology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, P.R. China广东省中医院[3]Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China[4]International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education (MOE), School of Pharmacy, Jinan University, Guangzhou, P.R. China
Wendan decoction, a classical traditional Chinese medicine formula consisting of six herbal medicines, has been widely used in clinical treatments for thousands of years due to the expectorant effects. However, the chemical basis of Wendan decoction remains unclear, which hinders the elucidation of the scientific connotation and mechanism of its effective components. In this study, an ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry method was first developed for characterization of its chemical profile, and a total of 142 chemical components including flavonoids, triterpenoids, alkaloids, coumarins, pungent phytochemicals, and other types were detected, among which 41 components were definitively identified with authentic standards. Furthermore, 14 major representative components were simultaneously quantified by high-performance liquid chromatography with ultraviolet detector, indicating that the content levels of flavonoids were the most abundant in Wendan decoction. In summary, this study established sensitive and practical methods to systematically characterize chemical profile for the first time and simultaneous quantify representative components of Wendan decoction. These findings above would provide a solid chemical basis for disclosure of potential effective components by further in vivo disposal study, and promote therapeutic mechanism researches of Wendan decoction.
基金：
This work was financially supported by the National Natural
Science Foundation of China (81774219); State Key Program
of National Natural Science Foundation of China
(81630097); National Major Scientific and Program of
Introducing Talents of Discipline to Universities (B13038);
the Key Project of First-class University Construction of
Guangzhou University of Chinese Medicine (XK2019024);
Dongguan Academician Workstation Project (DGYSZ-
2018-03).
第一作者机构：[1]College of Pharmacy, Jinan University, Guangzhou, P.R. China
共同第一作者：
通讯作者：
通讯机构：[1]College of Pharmacy, Jinan University, Guangzhou, P.R. China[3]Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, P. R. China[4]International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education (MOE), School of Pharmacy, Jinan University, Guangzhou, P.R. China[*1]College of Pharmacy, Jinan University,Guangzhou 510632, P.R.China.[*2]Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, P.R.China
推荐引用方式(GB/T 7714)：
Qi Wang,Zhenyu Zou,Yezi Zhang,et al.Characterization of chemical profile and quantification of major representative components of Wendan decoction, a classical traditional Chinese medicine formula[J].JOURNAL OF SEPARATION SCIENCE.2021,44(5):doi:10.1002/jssc.202000952.
APA：
Qi Wang,Zhenyu Zou,Yezi Zhang,Pei Lin,Taohua Lan...&Zhihong Yao.(2021).Characterization of chemical profile and quantification of major representative components of Wendan decoction, a classical traditional Chinese medicine formula.JOURNAL OF SEPARATION SCIENCE,44,(5)
MLA：
Qi Wang,et al."Characterization of chemical profile and quantification of major representative components of Wendan decoction, a classical traditional Chinese medicine formula".JOURNAL OF SEPARATION SCIENCE 44..5(2021)
