CircASH2L facilitates tumor-like biologic behaviours and inflammation of fibroblast-like synoviocytes via miR-129-5p/HIPK2 axis in rheumatoid arthritis.
机构：[1]Department of Nephrology, Western Theater General Hospital, Chengdu, Sichuan, China.[2]Department of Pathology, Weifang Traditional Chinese Hospital, Weifang, Shandong, China.[3]Department of Ophthalmology, Weifang Eye Hospital, Weifang, Shandong, China.[4]Department of Critical Care Medicine, Traditional Chinese Medicine University of Guangzhou First Affiliated Hospital, Guangzhou, Guangdong, China.[5]Department of Rheumatism, Dongying City People's Hospital, No. 317, Nanyi Road, Dongying City, Shandong Province, China.
Previous study showed that circular RNA Absent-Small-Homeotic-2--Like protein (circASH2L) was higher in rheumatoid arthritis (RA) patients. However, the roles and mechanisms of circASH2L in RA progression remain unclear.
Levels analysis was conducted using western blot and qRT-PCR. The proliferation, apoptosis, cell cycle progression, migration, invasiveness, and inflammation of RA fibroblast-like synoviocytes (RA-FLSs) were determined via MTT, flow cytometry, western blot, transwell, and ELISA assays.
CircASH2L knockdown in RA-FLSs suppressed cell proliferative, migratory, and invasive capacities, triggered cell cycle arrest, promoted apoptosis, and inhibited inflammation. Mechanistically, circASH2L targeted miR-129-5p, and repression of miR-129-5p abolished the functions of circASH2L silencing on the growth, motility, and inflammation of RA-FLSs. Besides, miR-129-5p was found to directly target HIPK2, and suppressed the tumor-like biologic behaviors and inflammation of RA-FLSs via regulating HIPK2. Importantly, we proved that circASH2L could modulate HIPK2 expression via miR-129-5p.
CircASH2L promoted RA-FLS growth, motility, and inflammation through miR-129-5p/HIPK2 axis.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|4 区医学
小类|4 区骨科
最新[2025]版：
大类|3 区医学
小类|3 区骨科
第一作者：
第一作者机构：[1]Department of Nephrology, Western Theater General Hospital, Chengdu, Sichuan, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Li Xia,Qu Meiting,Zhang Jie,et al.CircASH2L facilitates tumor-like biologic behaviours and inflammation of fibroblast-like synoviocytes via miR-129-5p/HIPK2 axis in rheumatoid arthritis.[J].Journal of orthopaedic surgery and research.2021,16(1):302.doi:10.1186/s13018-021-02432-3.
APA：
Li Xia,Qu Meiting,Zhang Jie,Chen Kuanyin&Ma Xianghui.(2021).CircASH2L facilitates tumor-like biologic behaviours and inflammation of fibroblast-like synoviocytes via miR-129-5p/HIPK2 axis in rheumatoid arthritis..Journal of orthopaedic surgery and research,16,(1)
MLA：
Li Xia,et al."CircASH2L facilitates tumor-like biologic behaviours and inflammation of fibroblast-like synoviocytes via miR-129-5p/HIPK2 axis in rheumatoid arthritis.".Journal of orthopaedic surgery and research 16..1(2021):302
