机构：[1]School of Pharmacy, Institute of Traditional Chinese Medicine and New Pharmacy Development, Guangdong Medical University, Dongguan, China[2]Central Research Laboratory, Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine, The Second People’s Hospital of Bao’an Shenzhen (Group) Shajing People’s Hospital of Bao’an Shenzhen, Shenzhen, China
Alzheimer's disease (AD) is a most common neurodegenerative disorder worldwide. Because of its complex pathogenesis, the prevention and therapies of AD still are a severe challenge. Evidence suggested that crocin, the major component of saffron, exhibited neuroprotective effects in AD. As such, in this study, N2a/APP695swe cells were enrolled to investigate the effects of crocin on endogenous Aβ-induced neurotoxicity. Crocin (100 and 200 μM) could ameliorate cytotoxicity according to CCK-8 assay and reduce apoptosis in line with Hoechst 33,342 staining and Annexin V-FITC/PI double staining in N2a/APP695swe cells. Reduced ROS generation and elevated MMP were found in N2a/APP695swe cells treated with crocin (100 and 200 μM). Additionally, crocin at concentrations of 100 and 200 μM inhibited the release of cytochrome and attenuated caspases-3 activity in N2a/APP695swe cells. Furthermore, succinylation, crotonylation, 2-hydroxyisobutyrylation, malonylation, and phosphorylation were significantly reduced, while a slight increase of acetylation was found in 100-μM crocin treated N2a/APP695swe cells. Taken together, crocin may be a promising natural product candidate for the effective cure of AD.
基金：
This work was supported by the Guangdong
Natural Science Foundation (No. 2018A030313084), Administration
of Traditional Chinese Medicine of Guangdong Province of China
(20191187&20192042), Medical Scientific Research Foundation
of Guangdong Province (A2021224), the Public Service Platform
Open Project Fund of South China Sea for R&D Marine Biomedicine
Resources (2HC18010), and Project of Educational Commission of
Guangdong Province (2020KTSCX047), Fund of Guangdong Medical
University (GDMUZ2020005).
第一作者机构：[1]School of Pharmacy, Institute of Traditional Chinese Medicine and New Pharmacy Development, Guangdong Medical University, Dongguan, China[2]Central Research Laboratory, Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine, The Second People’s Hospital of Bao’an Shenzhen (Group) Shajing People’s Hospital of Bao’an Shenzhen, Shenzhen, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Jikun Du,Yuanhua Li,Daibo Song,et al.Protective effects of crocin against endogenous Aβ-induced neurotoxicity in N2a/APP695swe cells.[J].PSYCHOPHARMACOLOGY.2021,238(10):2839-2847.doi:10.1007/s00213-021-05899-4.
APA：
Jikun Du,Yuanhua Li,Daibo Song,Jierong Liu,Qunfa Huang...&Li Li.(2021).Protective effects of crocin against endogenous Aβ-induced neurotoxicity in N2a/APP695swe cells..PSYCHOPHARMACOLOGY,238,(10)
MLA：
Jikun Du,et al."Protective effects of crocin against endogenous Aβ-induced neurotoxicity in N2a/APP695swe cells.".PSYCHOPHARMACOLOGY 238..10(2021):2839-2847
