Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma.
机构：[1]Department of Gastroenterology, Hubei Provincial Hospital of Integrated Chinese and Western Medicine, Wuhan 430015, Hubei, China[2]Department of Preventive Medicine, Tianhe District Hospital of Traditional Chinese Medicine, Guangzhou 510665, Guangdong, China
Gastric adenocarcinoma (GAC) is a common malignant tumor, accounting for 95% of gastric cancers. However, the effects and regulatory mechanisms of long non-coding RNA TRPM2-AS (TRPM2-AS) in GAC have not been fully explored. Our study investigates the action mechanism of TRPM2-AS in GAC. After performing quantitative Real-Time polymerase chain reaction or western blotting, we found that the levels of TRPM2-AS and Plasminogen Activator, Urokinase (PLAU) were upregulated in GAC, whereas the level of miR-138-5p was downregulated. Cell function experiments proved that silencing TRPM2-AS suppressed proliferation and migration and induced apoptosis in GAC cells. Bioinformatic analysis and luciferase assay identified the interaction between TRPM2-AS, miR-138-5p, and PLAU. In addition, the inhibitory effect of silencing TRPM2-AS on GAC cells could be partially relieved by PLAU overexpression. In conclusion, our study revealed that TRPM2-AS sponging miR-138-5p to upregulate PLAU could contribute to GAC progression, which might be useful for identifying biomarkers for GAC therapy.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|4 区生物
小类|4 区生物工程与应用微生物
最新[2025]版：
大类|4 区生物学
小类|4 区生物工程与应用微生物
第一作者：
第一作者机构：[1]Department of Gastroenterology, Hubei Provincial Hospital of Integrated Chinese and Western Medicine, Wuhan 430015, Hubei, China
共同第一作者：
通讯作者：
通讯机构：[2]Department of Preventive Medicine, Tianhe District Hospital of Traditional Chinese Medicine, Guangzhou 510665, Guangdong, China[*1]Department of Preventive Medicine, Tianhe District Hospital of Traditional Chinese Medicine, No.9, Zhongtangshi Road, Huangpu Avenue, Tianhe District, Guangzhou 510665, Guangdong, China
推荐引用方式(GB/T 7714)：
Sun Jun,Zhou Fang,Xue Juan,et al.Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma.[J].Bioengineered.2021,12(2):9753-9765.doi:10.1080/21655979.2021.1995101.
APA：
Sun Jun,Zhou Fang,Xue Juan,Ji Chunyan,Qu Yinzong&Pan Yuwei.(2021).Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma..Bioengineered,12,(2)
MLA：
Sun Jun,et al."Long non-coding RNA TRPM2-AS regulates microRNA miR-138-5p and PLAU (Plasminogen Activator, Urokinase) to promote the progression of gastric adenocarcinoma.".Bioengineered 12..2(2021):9753-9765
