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The effects of H22 tumor on the quality of oocytes and the development of early embryos from host mice: A single-cell RNA sequencing approach.

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资源类型：
Pubmed体系：
作者：
Yanhong Yang[a] * ; Xueying Zhang[b,c] * ; Yuting Lei[b] * ; Gang Chang[d] * ; Yan Zou[e] ; Siping Yu[b,c] ; Huijuan Wu[b,c] ; Hedong Rong[b,c] ; Zili Lei[b,*1] ; Changlong Xu[e,*2] ;
机构： [a]The First Affiliated Hospital (School of Clinical Medicine), Guangdong Pharmaceutical University, Nong-Lin-Xia Road 19#, Yue-Xiu District, Guangzhou, 510080, PR China [b]Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine, Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China, Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangdong TCM Key Laboratory for Metabolic Diseases, Guangzhou, 510006, PR China [c]School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, 510006, PR China [d]Department of Biochemistry and Molecular Biology, Shenzhen University Health Science Center, Shenzhen, 518060, PR China [e]The Reproductive Medical Center of Nanning Second People's Hospital, Nanning, 530031, PR China
出处：
DOI： 10.1016/j.theriogenology.2021.11.006
ISSN：

关键词： H22 Oocyte Chromosome Demethylation Zygote Single-cell RNA sequencing

摘要：
The association between cancer and female reproduction remains largely unknown. Here we investigated the quality of oocytes and the developmental potential of zygotes using H22 tumor-bearing mice model. The results showed that the number of oocytes was decreased in tumor-bearing mice compared with the control mice, and accompanied scattered chromosomes was observed. Further study revealed an abnormal epigenetic reprogramming occurred in the zygotes from the H22 tumor-bearing mice, as exemplified by the aberrant 5hmC/5mC modifications in the pronuclei. Finally, single-cell RNA sequencing was performed on the oocytes collected from the H22 tumor-bearing mice. Our data showed that 45 of the 202 differentially expressed genes in tumor-bearing group were closely associated with oocyte quality. Protein interaction analysis indicated that the potential interaction among these 45 genes. Collectively, our study uncovered that the quality of oocytes and early embryonic development were affected by H22 tumor bearing via the altered expression patterns of genes related with reproduction, providing new insights into the reproductive capability of female cancer patients.Copyright © 2021 Elsevier Inc. All rights reserved.

基金：
This work was supported by Guangxi Natural Science Fundation (No.2020GXNSFAA159099); the Science and Technology Project of Yue-Xiu District of Guangzhou City (No. 2018-WS-011), the Science and Technology Project of Qing-Xiu District of Nanning City (2020025); the Opening Foundation of the Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences (KLRB201807); the Science and Technology Project of Jiang-Nan District of Nanning City (2020011206); and the Key Research and Development Plan of Scientific Research and Technology in Liang-Qing District of Nanning City (202009).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类 | 2 区 农林科学
小类 | 1 区 兽医学 2 区 生殖生物学
最新[2025]版：
大类 | 2 区 农林科学
小类 | 1 区 兽医学 3 区 生殖生物学
第一作者：
第一作者机构： [a]The First Affiliated Hospital (School of Clinical Medicine), Guangdong Pharmaceutical University, Nong-Lin-Xia Road 19#, Yue-Xiu District, Guangzhou, 510080, PR China
共同第一作者：
通讯作者：
通讯机构： [b]Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine, Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education of China, Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangdong TCM Key Laboratory for Metabolic Diseases, Guangzhou, 510006, PR China [e]The Reproductive Medical Center of Nanning Second People's Hospital, Nanning, 530031, PR China [*1]Guangdong Metabolic Disease Research Center of Integrated Chinese and Western Medicine, Guangdong TCM key Laboratory for Metabolic Disease, Guangdong Pharmaceutical University, Wai-Huan-Dong Road 280#, Guangzhou Higher Education Mega Center, Guangzhou, 510006, PR China. [*2]IVF-LAB of the Reproductive Medical Center of Nanning Second People's Hospital, Nanning, 530031, China.
推荐引用方式(GB/T 7714)：
Yanhong Yang,Xueying Zhang,Yuting Lei,et al.The effects of H22 tumor on the quality of oocytes and the development of early embryos from host mice: A single-cell RNA sequencing approach.[J].Theriogenology.2022,179:45-59.doi:10.1016/j.theriogenology.2021.11.006.
APA：
Yanhong Yang,Xueying Zhang,Yuting Lei,Gang Chang,Yan Zou...&Changlong Xu.(2022).The effects of H22 tumor on the quality of oocytes and the development of early embryos from host mice: A single-cell RNA sequencing approach..Theriogenology,179,
MLA：
Yanhong Yang,et al."The effects of H22 tumor on the quality of oocytes and the development of early embryos from host mice: A single-cell RNA sequencing approach.".Theriogenology 179.(2022):45-59

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