机构：[1]College of Pharmacy, Gannan Medical University, Ganzhou 341000, China.[2]Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China.[3]Department of Medical Imaging, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China.[4]College of Rehabilitation, Gannan Medical University, Ganzhou 341000, China.[5]Key Laboratory of Traditional Chinese Medicine and New Pharmacy Development, Guangdong Medical University, Dongguan 523808, China.
Overexposure to glucocorticoid (GC) produces various clinical complications, including osteoporosis (OP), dyslipidemia, and hypercholesterolemia. Geniposide (GEN) is a natural iridoid compound isolated from Eucommia ulmoides. Our previous study found that GEN could alleviate dexamethasone (DEX)-induced differentiation inhibition of MC3T3-E1 cells. However, whether GEN protected against Dex-induced cholesterol accumulation in osteoblasts was still unclear.DEX was used to induce rat OP. Micro-CT data was obtained. The ALP activity and mineralization were determined by the staining assays, and the total intracellular cholesterol was determined by the ELISA kits. The protein expression was detected by western blot.GEN ameliorated Dex-induced micro-structure damages and cell differentiation inhibition in the bone trabecula in rats. In MC3T3-E1 cells, Dex enhanced the total intracellular cholesterol, which reduced the activity of cell proliferation and differentiation. Effectively, GEN decreased DEX-induced cholesterol accumulation, enhanced cell differentiation, and upregulated the expression of the GLP-1R/ABCA1 axis. In addition, inhibition of ABAC1 expression reversed the actions of GEN. Treatment with Exendin9-39, a GLP-1R inhibitor, could abrogate the protective activity of GEN.GEN ameliorated Dex-induced accumulation of cholesterol and inhibition of cell differentiation by mediating the GLP-1R/ABCA1 axis in MC3T3-E1 cells.
基金：
National Natural Science Foundation of China, grant
number 82060407, 81860261, 81860388, and 81960883; the Natural Science Foundation of Jiangxi
Province, grant number 20212ACB206002, 20202BABL206122, and 20202BABL206134; Scientific
Research Fund of Jiangxi Provincial Education Department, grant number GJJ201501 and GJJ201510;
Innovative Teamwork Project of Gannan Medical University, grant number TD201707.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|2 区生物
小类|3 区细胞生物学
最新[2025]版：
大类|2 区生物学
小类|3 区细胞生物学
第一作者：
第一作者机构：[1]College of Pharmacy, Gannan Medical University, Ganzhou 341000, China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zheng Yizhou,Xiao Yaosheng,Zhang Di,et al.Geniposide Ameliorated Dexamethasone-Induced Cholesterol Accumulation in Osteoblasts by Mediating the GLP-1R/ABCA1 Axis.[J].Cells.2021,10(12):doi:10.3390/cells10123424.
APA：
Zheng Yizhou,Xiao Yaosheng,Zhang Di,Zhang Shanshan,Ouyang Jing...&Wu Longhuo.(2021).Geniposide Ameliorated Dexamethasone-Induced Cholesterol Accumulation in Osteoblasts by Mediating the GLP-1R/ABCA1 Axis..Cells,10,(12)
MLA：
Zheng Yizhou,et al."Geniposide Ameliorated Dexamethasone-Induced Cholesterol Accumulation in Osteoblasts by Mediating the GLP-1R/ABCA1 Axis.".Cells 10..12(2021)
