机构：[1]Orthopedics Department, Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, China.[2]The Fifth Clinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China
The infrapatellar fat pad (IPFP) and synovium are reported to act as a functional unit, with emerging roles in the pathophysiology of knee osteoarthritis (KOA). Circular RNAs (circRNAs) are involved in the pathogenesis of KOA, especially in cartilage homeostasis regulation. Nevertheless, the regulatory mechanisms of circRNAs in the KOA IPFP/synovium unit remain to be elucidated. Therefore, the current study aimed to investigate alterations in the expression of circRNAs and predict their functions in the KOA IPFP/synovium unit using bioinformatics analysis.In brief, 6 synovium and IPFP specimens were collected, in which 3 from patients with KOA and 3 from controls. Then, circRNA sequencing was conducted on 2 KOA synovium and IPFP specimens as well as 1 control to investigate the expression profiles of circRNAs. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Reactome signaling pathway analyses were employed to predict the functions of the differentially expressed circRNAs. Based on the miRNA sponge theory, we constructed a circRNA-miRNA network to predict the molecular regulatory mechanism of these circRNAs. Venn analysis was performed to confirm the circRNAs and miRNAs expressed in both synovium and IPFP. Real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was also used to validate the expression levels of circRNAs that were co-expressed in both synovium and IPFP.A total of 65 and 72 circRNAs were differentially expressed in KOA synovium and IPFP, respectively (fold change ≥2, P<0.05). After obtaining the parental genes of differentially expressed circRNAs, the top 10 enrichment GO entries, KEGG pathways, and Reactome pathways were annotated. Furthermore, hsa_circ_0005265 was found to be down-regulated in both synovium and IPFP, which was validated by qRT-PCR. The circRNA-miRNA network was created to annotate the probable regulatory mechanisms of the differentially expressed circRNAs, which consequently confirmed 2 target miRNAs (hsa-miR-6769b-5p and hsa-miR-1249-5p) associated with hsa_circ_0005265 in both synovium and IPFP.Our outcomes bring us closer to understanding the potential mechanism of the IPFP/synovium unit in the progression of KOA and finding new molecular targets for KOA therapy.2021 Annals of Translational Medicine. All rights reserved.
基金：
Science and Technology Planning Project of Guangzhou City of China (Grant Number: 202002030204), Medical Science and Technology Research Fund Project of Guangdong Province of China (Grant Number: A2021463), Natural Science Foundation of Guangdong Province of China (Grant Number: 2021A1515011545), and Science and Technology Planning Project of Guangdong Province of China (Grant Number: 2021B1111610007).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|3 区医学
小类|3 区医学：研究与实验3 区肿瘤学
最新[2025]版：
无
第一作者：
第一作者机构：[1]Orthopedics Department, Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, China.
共同第一作者：
通讯作者：
通讯机构：[1]Orthopedics Department, Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou, China.[*1]Orthopedics Department, Guangdong Second Traditional Chinese Medicine Hospital, Guangzhou 510095, China
推荐引用方式(GB/T 7714)：
Jiang Tao,Lu Yimin,Chen Zehua,et al.RNA sequencing reveals the circular RNA expression profiles of the infrapatellar fat pad/synovium unit.[J].Annals of translational medicine.2021,9(22):1685.doi:10.21037/atm-21-5739.
APA：
Jiang Tao,Lu Yimin,Chen Zehua,Lin Xiaodong,Zhang Jiayuan...&Liu Wengang.(2021).RNA sequencing reveals the circular RNA expression profiles of the infrapatellar fat pad/synovium unit..Annals of translational medicine,9,(22)
MLA：
Jiang Tao,et al."RNA sequencing reveals the circular RNA expression profiles of the infrapatellar fat pad/synovium unit.".Annals of translational medicine 9..22(2021):1685
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