机构：[1]Department of Traditional Chinese Medicine (TCM) Gynecology, Hangzhou Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, Hangzhou, China,[2]Zhejiang Chinese Medical University, Hangzhou, China,[3]Second Clinical Medical College, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China,广东省中医院[4]Department of Phytochemistry, School of Pharmacy, The Second Military Medical University, Shanghai, China
BackgroundMaternal oocyte aging is strongly contributing to age-related decline in female fertility. Coenzyme Q10 (CoQ10) exerts positive effects in improving aging-related deterioration of oocyte quality, but the exact mechanism is unclear. ObjectiveTo reveal the system-level mechanism of CoQ10's anti-aging effect on oocytes based on network pharmacology. MethodsThis study adopted a systems network pharmacology approach, including target identification, data integration, network and module construction, bioinformatics analysis, molecular docking, and molecular dynamics simulation. ResultA total of 27 potential therapeutic targets were screened out. Seven hub targets (PPARA, CAT, MAPK14, SQSTM1, HMOX1, GRB2, and GSR) were identified. Functional and pathway enrichment analysis indicated that these 27 putative targets exerted therapeutic effects on oocyte aging by regulating signaling pathways (e.g., PPAR, TNF, apoptosis, necroptosisn, prolactin, and MAPK signaling pathway), and are involved oxidation-reduction process, mitochondrion, enzyme binding, reactive oxygen species metabolic process, ATP binding, among others. In addition, five densely linked functional modules revealed the potential mechanisms of CoQ10 in improving aging-related deterioration of oocyte quality are closely related to antioxidant, mitochondrial function enhancement, autophagy, anti-apoptosis, and immune and endocrine system regulation. The molecular docking study reveals that seven hub targets have a good binding affinity towards CoQ10, and molecular dynamics simulation confirms the stability of the interaction between the hub targets and the CoQ10 ligand. ConclusionThis network pharmacology study revealed the multiple mechanisms involved in the anti-aging effect of CoQ10 on oocytes. The molecular docking and molecular dynamics simulation provide evidence that CoQ10 may act on these hub targets to fight against oocytes aging.
基金：
This work was financially supported through grants from the
National Natural Science Foundation of China (82004003), the
Project of Zhejiang Province Scientific Research Foundation
(2020ZA078), Science and Technology Projects of Zhejiang
Province (2019C03086), the Project of Zhejiang Province
Scientific Research Foundation (2022ZQ061), the Medical and
Health Science and Technology Plan of Zhejiang Province
(2021KY920), and Zhejiang Zhangqin Famous Traditional
ChineseMedicine Expert Inheritance Studio Project (GZS2017014).
第一作者机构：[1]Department of Traditional Chinese Medicine (TCM) Gynecology, Hangzhou Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, Hangzhou, China,
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Yang Liuqing,Wang Heng,Song SuJie,et al.Systematic Understanding of Anti-Aging Effect of Coenzyme Q10 on Oocyte Through a Network Pharmacology Approach[J].FRONTIERS IN ENDOCRINOLOGY.2022,13:doi:10.3389/fendo.2022.813772.
APA：
Yang, Liuqing,Wang, Heng,Song, SuJie,Xu, Hongbin,Chen, Yun...&Zhang, Qin.(2022).Systematic Understanding of Anti-Aging Effect of Coenzyme Q10 on Oocyte Through a Network Pharmacology Approach.FRONTIERS IN ENDOCRINOLOGY,13,
MLA：
Yang, Liuqing,et al."Systematic Understanding of Anti-Aging Effect of Coenzyme Q10 on Oocyte Through a Network Pharmacology Approach".FRONTIERS IN ENDOCRINOLOGY 13.(2022)
