机构：[1]Department of Nephrology, The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou City, Guangdong Province, China[2]Nephrotic Diagnosis And Treatment Center, The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan City, Shandong Province, China
Diabetic nephropathy (DN) is associated with inflammation. Platycodin D (PD) demonstrates anti-inflammatory activity. However, whether PD affects DN remains to be explored. Here, we aimed to discuss the role of PD in DN and its underlying mechanisms. High glucose (HG)-induced HK-2 cells were treated with PD, and cell viability was assessed using the Thiazolyl Blue Tetrazolium Bromide (MTT) assay. Ferroptosis-related factors such as lactate dehydrogenase (LDH) activity, lipid reactive oxygen species (ROS), iron (Fe2+) level, GSH level, and malondialdehyde (MDA) level were evaluated. Cell death was evaluated using the TUNEL assay. GPX4 expression was evaluated using Quantitative Real-time PCR (qRT-PCR) and Western blotting analysis. The results indicated that HG increased LDH activity, lipid ROS production, Fe2+ levels, and MDA levels and decreased GSH levels, suggesting that the HG condition induced ferroptosis. PD treatment inhibited ferroptosis in HG-induced cells, downregulated ACSL4 and TFR1 expression, and upregulated FTH-1 and SLC7A11 expression. PD reversed the effects of HG condition on cell death. Moreover, GPX4 expression was downregulated in HG-stimulated cells. Furthermore, we substantiated that PD suppressed ferroptosis by modulating GPX4 expression. In conclusion, PD inhibited ferroptosis in HG-induced HK-2 cells by upregulating GPX4 expression, suggesting that PD may be an effective drug for the clinical treatment of DN.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|4 区生物学
小类|4 区生物工程与应用微生物
最新[2025]版：
大类|4 区生物学
小类|4 区生物工程与应用微生物
第一作者：
第一作者机构：[1]Department of Nephrology, The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou City, Guangdong Province, China
通讯作者：
通讯机构：[2]Nephrotic Diagnosis And Treatment Center, The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan City, Shandong Province, China[*1]The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, No.1, Jingba Road, Jinan City, Shandong Province 250001, China
推荐引用方式(GB/T 7714)：
Huang Jinzhong,Chen Gangyi,Wang Jilei,et al.Platycodin D regulates high glucose-induced ferroptosis of HK-2 cells through glutathione peroxidase 4 (GPX4).[J].Bioengineered.2022,13(3):6627-6637.doi:10.1080/21655979.2022.2045834.
APA：
Huang Jinzhong,Chen Gangyi,Wang Jilei,Liu Shibin&Su Jing.(2022).Platycodin D regulates high glucose-induced ferroptosis of HK-2 cells through glutathione peroxidase 4 (GPX4)..Bioengineered,13,(3)
MLA：
Huang Jinzhong,et al."Platycodin D regulates high glucose-induced ferroptosis of HK-2 cells through glutathione peroxidase 4 (GPX4).".Bioengineered 13..3(2022):6627-6637
