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Circular RNA NF1-419 Inhibits Proliferation and Induces Apoptosis by Regulating Lipid Metabolism in Astroglioma Cells.

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资源类型：
Pubmed体系：
作者：
Ran Li[1,3,4] * ; Xiaocui Tang[2] * ; Changqiong Xu[1,4] * ; Yinrui Guo[2] ; Longkai Qi[2] ; Shan Li[1] ; Qiuyun Ren[4] ; Wu Jie[4,*1] ; Diling Chen[2] ;
机构： [1]Hunan Yueyang Maternal & Child Health-Care Hospital, No. 693 Baling Middle Road, Yueyang 414000, P.R. China [2]Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied MicrobiologySouthern China, Institute of Microbiology, Guangdong Academy of Sciences (Guang Dong Detection Center of Microbiology),Guangzhou 510070, P.R. China [3]Yueyang Hospital of Traditional Chinese Medicine, No. 269 FengqiaohuRoad, Yueyang 414000, P.R. China [4]Brain Function and Disease Laboratory, Shantou University Medical College,No. 22 Xinling Road, Shantou 515041, Guangdong Province, P.R. China
出处：
DOI： 10.2174/1574892816666210729125802
ISSN：

关键词： Circular RNA circNF1-419 lipid metabolism apoptosis U87 astroglioma cell Ganoderma lucidum

摘要：
Astroglioma is the most common primary tumor of the central nervous system. Currently, there is no effective treatment for astroglioma. In the present study, the extract (L3) from Ganoderma Lucidum (G. lucidum) was found to inhibit the growth of astroglioma U87 cells and change the expression of circular RNAs (circRNAs). One of these, including the circular NF1-419 (circNF1-419), was of interest because NF1 gene is a classic tumor suppressor gene.The functional role of circ-NF1-419 in the inhibition of astroglioma cells remains unknown. This study focuses on the role of circNF1-419 in functional abnormalities of U87 astroglioma cells and aims to elaborate on its regulatory mechanism.The circNF1-419 overexpressing U87 (U87-NF1-419) cells were constructed. We generated U87-NF1-419 to evaluate the role of circNF1-419 on cell cycle, apoptosis, proliferation, tumor growth and metabolic regulation. Finally, we used docking screening to identify compounds in G. lucidum extracts that target circ-419.U87-NF1-419 can promote cell apoptosis and regulate lipid metabolism through glycerophospholipid metabolism and retrograde endocannabinoid signaling. Further examinations revealed that the expression of metabolic regulators, such as L-type voltage-operated calcium channels (L-VOCC), phospholipase C-β3 (PLCβ3), Mucin1, cationic amino acid transporter 4 (CAT4), cationic amino acid transporter 1 (CAT1) and a kinase (PRKA) anchor protein 4 (AKAP4) was inhibited, while phosphatidylserine synthase 1 (PTDSS1) was enhanced in U87-NF1-419 cells. In vivo experiments showed that circNF1-419 inhibits tumor growth in BALB/C nude mice, and enhanced AKAP4 and PTDSS1 in tumor tissues. The virtual docking screening results supported that ganosporeric acid A, ganodermatriol, ganoderic acid B and α-D-Arabinofuranosyladenine in L3 could activate circNF1-419 in astroglioma treatment.This study indicated that circNF1-419 could be a therapeutic target for the clinical treatment of astroglioma. L3 from Ganoderma Lucidum (G. lucidum) could inhibit astroglioma growth by activating circNF1-419.Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.

基金：
This work was supported by the National Natural Science Foundation of China (81701086), the Guangdong Science and Technology Plan Projects (2016A050502032, 2016A020215022, 2015A020211021, 2019A050520003), the Guangzhou Science and Technology Plan Projects (201504281708257; 201604020009), the High-level Leading Talent Introduction Program of GDAS (2016GDASRC- 0102), the Nanyue Microbial Talents Cultivation Fund of Guangdong Institute of Microbiology (GDIMYET- 20140203), the Guangzhou Medical University Research Projects (2016C28), the Research Foundation of Guangdong Administration of Traditional Chinese Medicine (No. 20201175), the Hunan Provincial Natural Science Foundation of China (No. 2019JJ50631), the Hunan Health Commission Project (No. 202102081835), the Research Foundation of Hunan Administration of Traditional Chinese Medicine (No. 2021091), the Clinical Innovation Leading Scientific Research Projects of Hunan Science and Technology Department (No. 2020SK52603), the Hunan Health Commission Project (No. 202103101455) and the Hunan Provincial Clinical Medical Technology Demonstration Base Project (No. 2020SK4025).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类 | 4 区 医学
小类 | 4 区 肿瘤学 4 区 药学
最新[2025]版：
大类 | 4 区 医学
小类 | 4 区 肿瘤学 4 区 药学
第一作者：
第一作者机构： [1]Hunan Yueyang Maternal & Child Health-Care Hospital, No. 693 Baling Middle Road, Yueyang 414000, P.R. China [3]Yueyang Hospital of Traditional Chinese Medicine, No. 269 FengqiaohuRoad, Yueyang 414000, P.R. China [4]Brain Function and Disease Laboratory, Shantou University Medical College,No. 22 Xinling Road, Shantou 515041, Guangdong Province, P.R. China
共同第一作者：
通讯作者：
通讯机构： [4]Brain Function and Disease Laboratory, Shantou University Medical College,No. 22 Xinling Road, Shantou 515041, Guangdong Province, P.R. China [*1]he Brain Function and Disease Laboratory, Shantou University Medical College, No. 22 Xinling Road, Shantou 515041, Guangdong Province, P.R. China
推荐引用方式(GB/T 7714)：
Ran Li,Xiaocui Tang,Changqiong Xu,et al.Circular RNA NF1-419 Inhibits Proliferation and Induces Apoptosis by Regulating Lipid Metabolism in Astroglioma Cells.[J].Recent patents on anti-cancer drug discovery.2022,17(2):162-177.doi:10.2174/1574892816666210729125802.
APA：
Ran Li,Xiaocui Tang,Changqiong Xu,Yinrui Guo,Longkai Qi...&Diling Chen.(2022).Circular RNA NF1-419 Inhibits Proliferation and Induces Apoptosis by Regulating Lipid Metabolism in Astroglioma Cells..Recent patents on anti-cancer drug discovery,17,(2)
MLA：
Ran Li,et al."Circular RNA NF1-419 Inhibits Proliferation and Induces Apoptosis by Regulating Lipid Metabolism in Astroglioma Cells.".Recent patents on anti-cancer drug discovery 17..2(2022):162-177

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