机构：[1] Guangzhou Univ Chinese Med, Inst Med Clin 2, Guangzhou 510120, Guangdong, Peoples R China[2] Guangdong Prov Acad Chinese Med Sci, Guangzhou 510120, Guangdong, Peoples R China
The rhizomes of Smilax glabra have been used as both food and folk medicine in many countries for a long time. However, little research has been reported on polysaccharides of S. glabra. In the present study, two polysaccharide fractions, SGP-1 and SGP-2, were isolated from the rhizomes of S. glabra with the number average molecular weights of 1.72 x 10(2) kDa and 1.31 x 10(2) kDa, and the weight average molecular weights of 131 x 10(5) kDa and 1.18 x 10(5) kDa, respectively, and their mainly monosaccharide compositions were both galactose and rhamnose (2.5:1). Both SGP-1 and SGP-2 significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) from LPS-induced RAW 264.7 cells, as well as the mRNA expression of inducible nitric oxide synthase (iNOS),TNF-alpha and IL-6. Additionally, SGP-1 and SGP-2 repressed the extracellular signal-regulated kinase (ERK) and c-Jun NH2-terminal kinase (JNK). These findings strongly suggested polysaccharides were also the anti-inflammatory active ingredient for S. glabra, and the potential of SGP-1 and SGP-2 as the anti-inflammatory agents. (C) 2015 Published by Elsevier Ltd.
基金：
National Natural Science Foun-dation of Guangdong, China (S2013030011515), Special funds forTCM Science and Technology of Guangdong provincial hospital oftraditional Chinese medicine (YK2013B1N02 and YK2013B1N11).
第一作者机构：[1] Guangzhou Univ Chinese Med, Inst Med Clin 2, Guangzhou 510120, Guangdong, Peoples R China[2] Guangdong Prov Acad Chinese Med Sci, Guangzhou 510120, Guangdong, Peoples R China
通讯作者：
通讯机构：[1] Guangzhou Univ Chinese Med, Inst Med Clin 2, Guangzhou 510120, Guangdong, Peoples R China[*1]Guangzhou Univ Chinese Med, Inst Med Clin 2, Guangzhou 510120, Guangdong, Peoples R China
推荐引用方式(GB/T 7714)：
Lu Chuan-li,Zhu Wei,Wang Min,等.Polysaccharides from Smilax glabra inhibit the pro-inflammatory mediators via ERK1/2 and JNK pathways in LPS-induced RAW264.7 cells[J].CARBOHYDRATE POLYMERS.2015,122:428-436.
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