Curcumin Suppresses Tumor Growth and Angiogenesis in Human Glioma Cells Through Modulation of Vascular Endothelial Growth Factor/Angiopoietin-2/Thrombospondin-1 Signaling
机构：[1] Univ Chinese Med Guangzhou, Affiliated Hosp 2, Chinese Med Hosp Guangdong Prov, Dept Neurosurg, Guangzhou, Guangdong, Peoples R China[2] Uppsala Univ, IECNSIR, Dept Surg Sci Anesthesiol & Intens Care Med, Lab Cerebrovasc Res,Univ Hosp, SE-75421 Uppsala, Sweden
Purpose: To investigate the effect of curcumin on tumor growth and angiogenesis of human gliomas and identify the underlying molecular mechanisms. Methods: A mouse xenograft glioma model was established by subcutaneously inoculating tumor cell aggregates derived from the U87 cell line. Mice were treated with 0.01ml/g body weight of curcumin or saline. Tumor volume was measured. Microvessel density was assessed by CD34 immunostaining, and angiogenesis by immunohistochemical staining of vascular endothelial growth factor (VEGF), angiopoietin-2 (Ang-2) and thrombospondin 1 (TSP-1). Results: At 28 days after treatment, tumor weights in the curcumin-treated group were much smaller than in the control group (0.23 +/- 0.11g vs. 0.44 +/- 0.15g p < 0.05), resulting in a 45.8% inhibition of tumor growth. Curcumin also markedly inhibited microvessel density. Expression of VEGF and Ang-2 was inhibited by curcumin, whereas TSP-1 expression was up-regulated. Conclusion: This study shows that curcumin inhibits tumor growth by inhibiting VEGF/Ang-2/TSP-1-mediated angiogenesis in a xenograft glioma mouse model.
基金：
This work was supported by the Guangdong Science and
Technology Plan Program (0903123) and the Swedish Medical
Research Council (2710 HSS/AS), Stockholm, Sweden;
Swedish Strategic Research Foundation, Stockholm, Sweden
(HS/AS).
第一作者机构：[1] Univ Chinese Med Guangzhou, Affiliated Hosp 2, Chinese Med Hosp Guangdong Prov, Dept Neurosurg, Guangzhou, Guangdong, Peoples R China[*1]Uppsala Univ, Uppsala Univ Hosp, CNS Injury & Repair IECNSIR, Res, Frodingsgatan 12,Bldg 1228, SE-7541 Uppsala, Sweden[*2]Med Hosp Guangdong Prov, 111 Dade Rd, Guangzhou 510060, Guangdong, Peoples R China
通讯作者：
通讯机构：[*1]Uppsala Univ, Uppsala Univ Hosp, CNS Injury & Repair IECNSIR, Res, Frodingsgatan 12,Bldg 1228, SE-7541 Uppsala, Sweden[*2]Med Hosp Guangdong Prov, 111 Dade Rd, Guangzhou 510060, Guangdong, Peoples R China
推荐引用方式(GB/T 7714)：
Zhang Zhiqiang,Li Cong,Tan Qijia,等.Curcumin Suppresses Tumor Growth and Angiogenesis in Human Glioma Cells Through Modulation of Vascular Endothelial Growth Factor/Angiopoietin-2/Thrombospondin-1 Signaling[J].CNS & NEUROLOGICAL DISORDERS-DRUG TARGETS.2017,16(3):346-350.
