A Comparison Study on Polysaccharides Extracted from Atractylodes chinensis (DC.) Koidz. Using Different Methods: Structural Characterization and Anti-SGC-7901 Effect of Combination with Apatinib
机构：[1]School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China[2]The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510006, China广东省中医院[3]Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, Guangzhou 510006, China
For hundreds of years, Atractylodes chinensis (DC.) Koidz. (AK) has been widely used as a treatment for spleen and stomach diseases in China. The AK polysaccharides (AKPs) have been thought to be the important bioactive components. In this stud, the impacts of different extraction methods were analyzed. The differences between AKPs extracted by hot water extraction (HWE), AKPs extracted by ultrasonic extraction (UAE), and AKPs extracted by enzyme extraction (EAE) were compared in terms of yield, total carbohydrate content, molecular weight distribution, monosaccharide composition, and synergistic activity of the AKPs with apatinib were determined. The results indicated that the yield of the polysaccharide obtained from HWE was higher than that of UAE and EAE. However, activity assays indicated that UAE-AKPs and HWE-AKPs enhanced apoptosis of human gastric cancer cells (SGC-7901) treated with apatinib and UAE-AKPs showed the strongest synergistic activities. This is also in agreement with the fact that UAE-AKPs have a smaller molecular weight, β-configuration, and higher galactose content. These findings suggested that UAE is an efficient and environmentally friendly method for producing new polysaccharides from Atractylodes chinensis (DC.) Koidz. for the development of natural synergist and for the treatment of gastric cancer.
第一作者机构：[1]School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou 510006, China
通讯作者：
通讯机构：[2]The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510006, China[3]Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, Guangzhou 510006, China
推荐引用方式(GB/T 7714)：
Zhou Pingfan,Xiao Wanwan,Wang Xiaoshuang,et al.A Comparison Study on Polysaccharides Extracted from Atractylodes chinensis (DC.) Koidz. Using Different Methods: Structural Characterization and Anti-SGC-7901 Effect of Combination with Apatinib[J].MOLECULES.2022,27(15):doi:10.3390/molecules27154727.
APA：
Zhou Pingfan,Xiao Wanwan,Wang Xiaoshuang,Wu Yayun,Zhao Ruizhi&Wang Yan.(2022).A Comparison Study on Polysaccharides Extracted from Atractylodes chinensis (DC.) Koidz. Using Different Methods: Structural Characterization and Anti-SGC-7901 Effect of Combination with Apatinib.MOLECULES,27,(15)
MLA：
Zhou Pingfan,et al."A Comparison Study on Polysaccharides Extracted from Atractylodes chinensis (DC.) Koidz. Using Different Methods: Structural Characterization and Anti-SGC-7901 Effect of Combination with Apatinib".MOLECULES 27..15(2022)
