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Comparison of different extraction methods for polysaccharides from Dendrobium officinale stem.

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机构: [a]Guangdong Food and Drug Vocational College, Guangzhou, Guangdong, 510520, PR China [b]School of Pharmaceutical Science, and Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510006, PR China [c]Affiliated Huai’an Hospital of Xuzhou Medical University, Huai’an, Jiangsu, 223001, PR China [d]Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huai’an, Jiangsu, 223003, PR China [e]Dongguan Mathematical Engineering Academy of Chinese Medicine and Guangzhou University of Chinese Medicine, Dongguan, Guangdong, 523808, PR China
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关键词: Dendrobium officinale stem polysaccharide Extraction method Yield Characteristic Antioxidant activity

摘要:
The purpose of this study was to screen the optimum extraction of polysaccharides (DOP) from Dendrobium officinale stem. Firstly, different methods, including hot water extraction (HWE), cold-pressing (CP), freeze-thawing cold-pressing (FTCP), ultrasonic-assisted hot water extraction (UHWE), microwave-assisted hot water extraction (MHWE) and enzyme-assisted hot water extraction (EHWE), were employed to extract DOP under their respective best parameters. Then, the extraction yield, structure and antioxidant activity of the polysaccharides from different extraction methods were compared under the same condition. The data implied that UHWE and FTCP possessed higher extraction yield than the other extraction methods. Besides, DOPCP and DOPFTCP had higher molecular weight than the other polysaccharide samples. More importantly, DOPFTCP had the highest antioxidant activity. Overall, DOPFTCP exhibit high extraction yield, well-preserved molecular chains and best antioxidant activity, all these indicated FTCP was the most suitable method to extract DOP. Copyright © 2018 Elsevier Ltd. All rights reserved.

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出版当年[2017]版:
大类 | 2 区 化学
小类 | 1 区 应用化学 2 区 有机化学 2 区 高分子科学
最新[2025]版:
大类 | 1 区 化学
小类 | 1 区 应用化学 1 区 有机化学 1 区 高分子科学
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第一作者机构: [a]Guangdong Food and Drug Vocational College, Guangzhou, Guangdong, 510520, PR China [*1]Guangdong Food and Drug Vocational College, Guangzhou, Guangdong, 510520, PR China.
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通讯机构: [a]Guangdong Food and Drug Vocational College, Guangzhou, Guangdong, 510520, PR China [b]School of Pharmaceutical Science, and Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510006, PR China [e]Dongguan Mathematical Engineering Academy of Chinese Medicine and Guangzhou University of Chinese Medicine, Dongguan, Guangdong, 523808, PR China [*1]Guangdong Food and Drug Vocational College, Guangzhou, Guangdong, 510520, PR China. [*2]School of Pharmaceutical Science, and Guangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510006, PR China.
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