Maternal pre-pregnancy body mass index and offspring with overweight/obesity at preschool age: The possible role of epigenome-wide DNA methylation changes in cord blood
机构：[1]Huazhong Univ Sci & Technol, Sch Publ Hlth, Dept Maternal & Child Hlth, Tongji Med Coll, 13 Hangkong Rd, Wuhan, Peoples R China[2]Tradit Chinese Med Hosp, Zhuhai, Guangdong, Peoples R China[3]Zhuhai Ctr Dis Control & Prevent, Zhuhai, Guangdong, Peoples R China[4]Zhuhai Women & Childrens Hosp, Zhuhai, Guangdong, Peoples R China
Background Epigenome-wide association studies have identified some DNA methylation sites associated with body mass index (BMI) or obesity. Studies in the Asian population are lacking. Objective To examine the association of cord blood genome-wide DNA methylation (GWDm) changes with maternal pre-pregnancy BMI and children's BMI-z score at preschool age. Additionally, we also explored the genome-wide differentially methylated regions and differentially methylated probes between preschoolers with overweight/obesity and normal-weight counterparts. Methods This two-stage study design included (1) a GWDm analysis of 30 mother-child pairs from 633 participants of the Zhuhai birth cohort with data on newborn cord blood, maternal pre-pregnancy BMI, and children's BMI at 3 years of age; and (2) a targeted validation analysis of the cord blood of ten children with overweight/obesity and ten matched controls to validate the CpG sites. Results In the first stage, no significant CpG sites were found to be associated with children's BMI-z score at preschool age after FDR correction with the p-values of the CpG sites in FOXN3 (cg23501836) and ZNF264 (cg27437574) being close to 1 x 10(-6). In the second stage, a significant difference of CpG sites in AHRR (chr5:355067-355068) and FOXN3 (chr14: 89630264-89630272 and chr14: 89630387-89630388) was found between the ten children with overweight/obesity and ten controls (p < 0.05). The CpG sites in FOXN3 (chr14:89630264-89630272 and chr14:89630295-89630296) and ZNF264 (chr19: 57703104-57703107 and chr19: 57703301-57703307) were associated with children's BMI-z score; and the CpG sites in FOXN3 (chr14: 89630264-89630272 and chr14: 89630387-89630388) were associated with maternal pre-pregnancy BMI. Conclusions DNA methylation in FOXN3 and AHRR is associated with overweight/obesity in preschool-aged children, and the methylation in FOXN3 and ZNF264 might be associated with children's BMI-z score. FOXN3 methylation may be associated with maternal pre-pregnancy BMI, suggesting its potential role in the children's BMI-z score or overweight/obesity. Our results provide novel insights into the mechanisms of children's obesity.
基金：
Natural Science Foundation of China [81673182]; Zhuhai Municipal Science and Technology Bureau in Medical and Health Project [ZH22036201210183PWC]; Maternal and Child Health Hospital in Zhuhai city
第一作者机构：[1]Huazhong Univ Sci & Technol, Sch Publ Hlth, Dept Maternal & Child Hlth, Tongji Med Coll, 13 Hangkong Rd, Wuhan, Peoples R China
通讯作者：
通讯机构：[1]Huazhong Univ Sci & Technol, Sch Publ Hlth, Dept Maternal & Child Hlth, Tongji Med Coll, 13 Hangkong Rd, Wuhan, Peoples R China[*1]Department of Maternal and Child Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, China
推荐引用方式(GB/T 7714)：
Chang Ruixia,Zhang Yuanyuan,Sun Jiahong,et al.Maternal pre-pregnancy body mass index and offspring with overweight/obesity at preschool age: The possible role of epigenome-wide DNA methylation changes in cord blood[J].PEDIATRIC OBESITY.2023,18(1):doi:10.1111/ijpo.12969.
APA：
Chang, Ruixia,Zhang, Yuanyuan,Sun, Jiahong,Xu, Ke,Li, Chunan...&Zhang, Jianduan.(2023).Maternal pre-pregnancy body mass index and offspring with overweight/obesity at preschool age: The possible role of epigenome-wide DNA methylation changes in cord blood.PEDIATRIC OBESITY,18,(1)
MLA：
Chang, Ruixia,et al."Maternal pre-pregnancy body mass index and offspring with overweight/obesity at preschool age: The possible role of epigenome-wide DNA methylation changes in cord blood".PEDIATRIC OBESITY 18..1(2023)
