机构：[1]Department of Cardiovascular Surgery, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China[2]JNU-HKUST Joint Laboratory for Neuroscience and Innovative Drug Research, College of Pharmacy, Jinan University, Guangzhou 510632, China[3]Guangdong Provincial Engineering Research Center for Modernization of TCM, College of Pharmacy, Jinan University, Guangzhou 510632, China[4]Centro Hospitalar Conde de São Januário, Macau, China[5]NMPA Key Laboratory for Quality Evaluation of TCM, Jinan University, Guangzhou 510632, China
Here, (-)-Tetrahydroalstonine (THA) was isolated from Alstonia scholaris and investigated for its neuroprotective effect towards oxygen-glucose deprivation/re-oxygenation (OGD/R)-induced neuronal damage. In this study, primary cortical neurons were pre-treated with THA and then subjected to OGD/R induction. The cell viability was tested by the MTT assay, and the states of the autophagy-lysosomal pathway and Akt/mTOR pathway were monitored by Western blot analysis. The findings suggested that THA administration increased the cell viability of OGD/R-induced cortical neurons. Autophagic activity and lysosomal dysfunction were found at the early stage of OGD/R, which were significantly ameliorated by THA treatment. Meanwhile, the protective effect of THA was significantly reversed by the lysosome inhibitor. Additionally, THA significantly activated the Akt/mTOR pathway, which was suppressed after OGD/R induction. In summary, THA exhibited promising protective effects against OGD/R-induced neuronal injury by autophagy regulation through the Akt/mTOR pathway.
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外文
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中科院(CAS)分区：
出版当年[2022]版：
大类|2 区化学
小类|3 区化学：综合3 区生化与分子生物学
最新[2025]版：
大类|3 区化学
小类|3 区生化与分子生物学3 区化学：综合
第一作者：
第一作者机构：[1]Department of Cardiovascular Surgery, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China[2]JNU-HKUST Joint Laboratory for Neuroscience and Innovative Drug Research, College of Pharmacy, Jinan University, Guangzhou 510632, China
共同第一作者：
通讯作者：
通讯机构：[3]Guangdong Provincial Engineering Research Center for Modernization of TCM, College of Pharmacy, Jinan University, Guangzhou 510632, China[5]NMPA Key Laboratory for Quality Evaluation of TCM, Jinan University, Guangzhou 510632, China
推荐引用方式(GB/T 7714)：
Liao Yumei,Wang Jun-Ya,Pan Yan,et al.The Protective Effect of (-)-Tetrahydroalstonine against OGD/R-Induced Neuronal Injury via Autophagy Regulation[J].Molecules (Basel, Switzerland).2023,28(5):doi:10.3390/molecules28052370.
APA：
Liao Yumei,Wang Jun-Ya,Pan Yan,Zou Xueyi,Wang Chaoqun...&Zhang Shiqing.(2023).The Protective Effect of (-)-Tetrahydroalstonine against OGD/R-Induced Neuronal Injury via Autophagy Regulation.Molecules (Basel, Switzerland),28,(5)
MLA：
Liao Yumei,et al."The Protective Effect of (-)-Tetrahydroalstonine against OGD/R-Induced Neuronal Injury via Autophagy Regulation".Molecules (Basel, Switzerland) 28..5(2023)
化学