Liver Protection of a Low-Polarity Fraction from Ficus pandurata Hance, Prepared by Supercritical CO2 Fluid Extraction, on CCl4-Induced Acute Liver Injury in Mice via Inhibiting Apoptosis and Ferroptosis Mediated by Strengthened Antioxidation
机构：[1]Pharmacology Laboratory, Zhongshan Hospital, Guangzhou University of Chinese Medicine, Zhongshan 528401, China.深圳市中医院深圳医学信息中心[2]Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, And School of Pharmacy, Guangdong Medical University, Dongguan 523808, China.[3]Shenzhen Baoan Authentic TCM Therapy Hospital, Shenzhen 518101, China.[4]School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China.[5]Dongguan Key Laboratory of Chronic Inflammatory Diseases, The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan 523121, China.
Ficus pandurata Hance (FPH) is a Chinese herbal medicine widely used for health care. This study was designed to investigate the alleviation efficacy of the low-polarity ingredients of FPH (FPHLP), prepared by supercritical CO2 fluid extraction technology, against CCl4-induced acute liver injury (ALI) in mice and uncover its underlying mechanism. The results showed that FPHLP had a good antioxidative effect determined by the DPPH free radical scavenging activity test and T-AOC assay. The in vivo study showed that FPHLP dose-dependently protected against liver damage via detection of ALT, AST, and LDH levels and changes in liver histopathology. The antioxidative stress properties of FPHLP suppressed ALI by increasing levels of GSH, Nrf2, HO-1, and Trx-1 and reducing levels of ROS and MDA and the expression of Keap1. FPHLP significantly reduced the level of Fe2+ and expression of TfR1, xCT/SLC7A11, and Bcl2, while increasing the expression of GPX4, FTH1, cleaved PARP, Bax, and cleaved caspase 3. The results demonstrated that FPHLP protected mouse liver from injury induced by CCl4 via suppression of apoptosis and ferroptosis. This study suggests that FPHLP can be used for liver damage protection in humans, which strongly supports its traditional use as a herbal medicine.
基金：
National Natural Science Foundation
of China (81503303, 81973548, and 81473401), Discipline Construction Project of Guangdong Medical
University (4SG22009G and 4SG21009G), Guangdong Innovation Team Project for Regular Institutions of Higher Learning (2022KCXTD011), Guangdong Medical University Research Foundation
(4SG22242G), Traditional Chinese Medicine Bureau of Guangdong Province, China (20213011), Project
of Administration of Traditional Chinese Medicine of Guangdong Province of China (20182168), and
Special Fund for Science and Technology Development of Zhanjiang (2022A01003).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类|2 区化学
小类|3 区化学：综合3 区生化与分子生物学
最新[2025]版：
大类|3 区化学
小类|3 区生化与分子生物学3 区化学：综合
第一作者：
第一作者机构：[1]Pharmacology Laboratory, Zhongshan Hospital, Guangzhou University of Chinese Medicine, Zhongshan 528401, China.
共同第一作者：
通讯作者：
通讯机构：[2]Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, And School of Pharmacy, Guangdong Medical University, Dongguan 523808, China.[5]Dongguan Key Laboratory of Chronic Inflammatory Diseases, The First Dongguan Affiliated Hospital, Guangdong Medical University, Dongguan 523121, China.
推荐引用方式(GB/T 7714)：
Dai Weibo,Pang Xiaoyan,Peng Weiwen,et al.Liver Protection of a Low-Polarity Fraction from Ficus pandurata Hance, Prepared by Supercritical CO2 Fluid Extraction, on CCl4-Induced Acute Liver Injury in Mice via Inhibiting Apoptosis and Ferroptosis Mediated by Strengthened Antioxidation[J].Molecules (Basel, Switzerland).2023,28(5):doi:10.3390/molecules28052078.
APA：
Dai Weibo,Pang Xiaoyan,Peng Weiwen,Zhan Xinyi,Chen Chang...&Hu Xianjing.(2023).Liver Protection of a Low-Polarity Fraction from Ficus pandurata Hance, Prepared by Supercritical CO2 Fluid Extraction, on CCl4-Induced Acute Liver Injury in Mice via Inhibiting Apoptosis and Ferroptosis Mediated by Strengthened Antioxidation.Molecules (Basel, Switzerland),28,(5)
MLA：
Dai Weibo,et al."Liver Protection of a Low-Polarity Fraction from Ficus pandurata Hance, Prepared by Supercritical CO2 Fluid Extraction, on CCl4-Induced Acute Liver Injury in Mice via Inhibiting Apoptosis and Ferroptosis Mediated by Strengthened Antioxidation".Molecules (Basel, Switzerland) 28..5(2023)
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