机构：[1]The Tenth Affiliated Hospital of Southern Medical University, Dongguan, Guangdong, 523059, China.[2]The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong, 510515, China.[3]Guangdong Provincial Key Laboratory of Cardiac Function and Microcirculation, Guangzhou, 510515, China.[4]Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.深圳市中医院深圳医学信息中心[5]Department of Molecular Biomedical Sciences, North Carolina State University, Raleigh, NC, 27606, USA.[6]Cardiology Department of Panyu Central Hospital and Cardiovascular Disease Institute of Panyu District, Guangzhou, 511400, P. R. China.[7]College of Pharmaceutical Science, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Hebei University, Baoding, 071002, China.[8]Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.[9]Department of Biomedical Engineering, Columbia University, New York, 10032, USA.
The stromal-derived factor 1𝜶/chemokine receptor 4 (SDF-1 𝜶/CXCR4) axis
contributes to myocardial protection after myocardial infarction (MI) by
recruiting endogenous stem cells into the ischemic tissue. However,
excessive inflammatory macrophages are also recruited simultaneously,
aggravating myocardial damage. More seriously, the increased inflammation
contributes to abnormal cardiomyocyte electrical coupling, leading to
inhomogeneities in ventricular conduction and retarded conduction velocity. It
is highly desirable to selectively recruit the stem cells but block the
inflammation. In this work, SDF-1 𝜶-encapsulated Puerarin (PUE) hydrogel
(SDF-1 𝜶@PUE) is capable of enhancing endogenous stem cell homing and
simultaneously polarizing the recruited monocyte/macrophages into a
repairing phenotype. Flow cytometry analysis of the treated heart tissue
shows that endogenous bone marrow mesenchymal stem cells, hemopoietic
stem cells, and immune cells are recruited while SDF-1 𝜶@PUE efficiently
polarizes the recruited monocytes/macrophages into the M2 type. These
macrophages influence the preservation of connexin 43 (Cx43) expression
which modulates intercellular coupling and improves electrical conduction.
Furthermore, by taking advantage of the improved “soil”, the recruited stem
cells mediate an improved cardiac function by preventing deterioration,
promoting neovascular architecture, and reducing infarct size. These findings
demonstrate a promising therapeutic platform for MI that not only facilitates
heart regeneration but also reduces the risk of cardiac arrhythmias.
基金：
National Key R&D Program of
China (2022YFB3808300). National Natural Science Foundation of
China (32322045, 32271420, 21977024, 82202339, 82202307, and
22207050). Guangdong Basic and Applied Basic Research Foundation
(2021B1515120065). All animal studies were approved by the Dongguan
People’s Hospital Ethics Committee (IACUC-202209001), the Experimental Animal Ethics Committee of Hebei University (IACUC-2020XG053) and
were conducted according to the Regulations for the Administration of Affairs Concerning Experimental Animals (P. R. China); all animal studies of electrophysiological experiments with rats were approved by Scope
Research Institute of electrophysiology Application for Laboratory Animal
Welfare and Ethical Committee (SGLL20220716051), and were conducted
according to the Regulations for the Administration of Affairs Concerning
Experimental Animals (P. R. China).
第一作者机构：[1]The Tenth Affiliated Hospital of Southern Medical University, Dongguan, Guangdong, 523059, China.[2]The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong, 510515, China.[3]Guangdong Provincial Key Laboratory of Cardiac Function and Microcirculation, Guangzhou, 510515, China.
通讯作者：
通讯机构：[1]The Tenth Affiliated Hospital of Southern Medical University, Dongguan, Guangdong, 523059, China.[2]The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong, 510515, China.[3]Guangdong Provincial Key Laboratory of Cardiac Function and Microcirculation, Guangzhou, 510515, China.
推荐引用方式(GB/T 7714)：
Luo Li,Li Yuetong,Bao Ziwei,et al.Pericardial Delivery of SDF-1 𝜶 Puerarin Hydrogel Promotes Heart Repair and Electrical Coupling[J].Advanced materials (Deerfield Beach, Fla.).2023,e2302686.doi:10.1002/adma.202302686.
APA：
Luo Li,Li Yuetong,Bao Ziwei,Zhu Dashuai,Chen Guoqin...&Li Zhenhua.(2023).Pericardial Delivery of SDF-1 𝜶 Puerarin Hydrogel Promotes Heart Repair and Electrical Coupling.Advanced materials (Deerfield Beach, Fla.),,
MLA：
Luo Li,et al."Pericardial Delivery of SDF-1 𝜶 Puerarin Hydrogel Promotes Heart Repair and Electrical Coupling".Advanced materials (Deerfield Beach, Fla.) .(2023):e2302686
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