The Metabolism Study of Oleraisoquinoline in Rats Using Ultrahigh-performance Liquid Chromatography-electrospray Coupled with Quadrupole Time-of-flight Mass Spectrometry and its Bioactivities
机构：[1]Liaoning Univ Tradit Chinese Med, Sch Pharm, 77 Shengming 1 Rd, Dalian 116600, Peoples R China[2]Liaoning Univ Tradit Chinese Med, Sch Informat Engn, Shenyang, Liaoning, Peoples R China[3]Guangzhou Univ Chinese Med, Guangdong Prov Hosp Chinese Med, Guangdong Prov Acad Chinese Med Sci, State Key Lab Tradit Chinese Med Syndrome,Affiliat, Guangzhou 510006, Guangdong, Peoples R China广东省中医院
Objective: This study aimed to investigate the main metabolites and metabolic pathways of oleraisoquinoline in rats, a new alkaloid isolated from Portulaca oleracea L., and test its antioxidation and anticholinesterase effects.Methods: Ultra-high-performance liquid chromatography-electrospray coupled with quadrupole time-of-flight mass spectrometry (UHPLC-ESI-Q-TOF/MS) was applied to study the metabolism of oleraisoquinoline. Furthermore, 1,1-diphenyl-2-picrylhydrazyl assay and modified Ellman's method were used to test the antioxidation and anticholinesterase effects of oleraisoquinoline, respectively.Results: The metabolism results of oleraisoquinoline showed, after its administration through the tail vein of rats, 4 metabolites in the plasma samples, 17 metabolites in the urine sample, and 2 metabolites in the feces sample. The main metabolic pathways were hydrolyzation, oxidation, hydroxylation, sulfonation, glucuronidation, acetylation, and methylation. Additionally, IC50 values of antioxidant and anticholinesterase activities were 13.819 +/- 0.005 mu M and 10.551 +/- 0.069 mu M, respectively.Conclusion: 21 metabolites were found in the rat's plasma, urine, and feces samples, and the metabolic pathways included hydrolyzation, oxidation, hydroxylation, sulfonation, glucuronidation, acetylation, and methylation; among them, sulfonation was the main metabolic reaction. Meanwhile, oleraisoquinoline also showed extremely good antioxidant and anticholinesterase activities.
基金：
National Natural Science Foundation of China [82073990]
第一作者机构：[1]Liaoning Univ Tradit Chinese Med, Sch Pharm, 77 Shengming 1 Rd, Dalian 116600, Peoples R China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Liu Peishan,Sun Rui,Tian Jiayin,et al.The Metabolism Study of Oleraisoquinoline in Rats Using Ultrahigh-performance Liquid Chromatography-electrospray Coupled with Quadrupole Time-of-flight Mass Spectrometry and its Bioactivities[J].CURRENT PHARMACEUTICAL ANALYSIS.2023,19(9):695-703.doi:10.2174/1573412919666230816090927.
APA：
Liu, Peishan,Sun, Rui,Tian, Jiayin,He, Fan&Ying, Xixiang.(2023).The Metabolism Study of Oleraisoquinoline in Rats Using Ultrahigh-performance Liquid Chromatography-electrospray Coupled with Quadrupole Time-of-flight Mass Spectrometry and its Bioactivities.CURRENT PHARMACEUTICAL ANALYSIS,19,(9)
MLA：
Liu, Peishan,et al."The Metabolism Study of Oleraisoquinoline in Rats Using Ultrahigh-performance Liquid Chromatography-electrospray Coupled with Quadrupole Time-of-flight Mass Spectrometry and its Bioactivities".CURRENT PHARMACEUTICAL ANALYSIS 19..9(2023):695-703
