机构：[1]Wadsworth Center, New York State Department of Health, Empire State Plaza, PO Box 509, Albany, NY 12201-0509, USA[2]University at Albany, State University of New York, 1400 Washington Avenue, Albany, NY 12222, USA[3]Yale University School of Medicine, 333 Cedar St, New Haven, CT 06510, USA[4]The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, 111 Dade Road, Guangzhou 510120, China大德路总院广东省中医院深圳市中医院深圳医学信息中心
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for measurements of steroids in human saliva has garnered increased interest in the area of clinical psychoneuroendocrinological research. However, performance characteristics of LC-MS/MS methods for the analysis of steroids in saliva are limited. Human saliva samples were collected via passive drool. Cortisol and dehydroepiandrosterone sulfate (DHEA-S) in the samples were extracted together, resolved on a C18-A column, and analyzed using tandem mass spectrometry. The LC-MS/MS method had limits of quantitation of 0.03 and 0.06ng/mL for DHEA-S and cortisol, respectively. Method evaluations showed coefficient variation (%CV) of inter-assay ranging 4.6-17.9% for DHEA-S and cortisol, recoveries of 102.4-109.5% for DHEA-S and 94.6-98.3% for cortisol, and assay linearity with R-2=0.9964 for DHEA-S (1.0-25.0ng/mL) and R-2=0.997 (1.0-25.0ng/mL) for cortisol. No cross contamination among samples was observed. Human saliva showed 20% and 18% ion enhancement effect for DHEA-S and cortisol assay, respectively. No interference by ten common steroids was detected. Regression analysis of method comparisons with laboratory-developed test (LDT) method revealed R-2=0.9688 (LC-MS/MS=0.9665 LDT-LC-MS/MS-0.7355) for cortisol, and R-2=0.9039 (LC-MS/MS=1.0173 LDT-LC-MS/MS+3.6797) for DHEA-S. Reference ranges for young adults were determined to be 0.3-5.9ng/mL for females and 0.1-5.6ng/mL for males for salivary cortisol, and 0.6-7.4ng/mL for females and 0.6-10.1ng/mL for males for salivary DHEA-S. An LC-MS/MS method for quantifying cortisol and DHEA-S in human saliva was developed and validated for clinical and psychoneuroendocrinological research that require noninvasive means of measuring these hormones.
基金：
National Institute on Drug AbuseUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute on Drug Abuse (NIDA) [F31 DA038931]
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区化学
小类|2 区分析化学3 区生化研究方法
最新[2025]版：
大类|2 区化学
小类|3 区生化研究方法3 区分析化学
JCR分区：
出版当年[2017]版：
Q1CHEMISTRY, ANALYTICALQ2BIOCHEMICAL RESEARCH METHODS
最新[2023]版：
Q1BIOCHEMICAL RESEARCH METHODSQ1CHEMISTRY, ANALYTICAL
第一作者机构：[1]Wadsworth Center, New York State Department of Health, Empire State Plaza, PO Box 509, Albany, NY 12201-0509, USA[2]University at Albany, State University of New York, 1400 Washington Avenue, Albany, NY 12222, USA
通讯作者：
通讯机构：[1]Wadsworth Center, New York State Department of Health, Empire State Plaza, PO Box 509, Albany, NY 12201-0509, USA[2]University at Albany, State University of New York, 1400 Washington Avenue, Albany, NY 12222, USA
推荐引用方式(GB/T 7714)：
Cao Zhimin (Tim),Wemm Stephanie E.,Han Liqiao,et al.Noninvasive determination of human cortisol and dehydroepiandrosterone sulfate using liquid chromatography-tandem mass spectrometry[J].ANALYTICAL AND BIOANALYTICAL CHEMISTRY.2019,411(6):1203-1210.doi:10.1007/s00216-018-1549-x.
APA：
Cao, Zhimin (Tim),Wemm, Stephanie E.,Han, Liqiao,Spink, David C.&Wulfert, Edelgard.(2019).Noninvasive determination of human cortisol and dehydroepiandrosterone sulfate using liquid chromatography-tandem mass spectrometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,411,(6)
MLA：
Cao, Zhimin (Tim),et al."Noninvasive determination of human cortisol and dehydroepiandrosterone sulfate using liquid chromatography-tandem mass spectrometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411..6(2019):1203-1210
