机构：[1]Department of Neurology, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000 P.R. China[2]Joint Laboratory for Translational Medicine Research, Beijing Institute of Genomics, Chinese Academy of Sciences & Liaocheng People's Hospital, CAS Key Laboratory of Genomic Science and Information, Chinese Academy of Sciences, Beijing 100101 P.R. China[3]Joint Laboratory for Translational Medicine Research, Beijing Institute of Genomics, Chinese Academy of Sciences & Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China[4]Taishan Medical University, Taian, Shandong 271016 P.R. China[5]Department of Ultrasonic, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000 P.R. China[6]Deparment of Neurology, Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou, Guangdong 510120 P.R. China广东省中医院[7]Department of Neurosurgery, Liaocheng People’s Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000 PR China[8]Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021 P.R. China
Atherosclerosis (AS) is a multifactorial disease. Exploration of DNA methylation in regulating gene transcription in a cell type-and stage-specific manner will shed light on understanding the biological processes associated with plaque stability. We identified 174 up-regulated genes with hypo-methylation in the promoter, and 86 down-regulated genes with hyper-methylation in the promoter, in AS vs. healthy controls. Among them, high expression of signaling lymphocytic activation molecule 7 (SLAM7) was examined in carotid plaque vs. intact tissue, in advanced plaque vs. early atherosclerotic tissue, and SLAMF7 protein expressed significantly higher in the unstable plaques than that in the stable plaques, especially in the CD68-positive macrophages. Depletion of SLAMF7 in plaque-derived macrophages induced a suppressed secretion of proinflammatory cytokines, and inhibited proliferation of vascular smooth muscle cells. These data provide emerging evidence that SLAMF7 could be a target of potential therapeutic intervention in carotid AS.
基金：
This work was supported by the Natural Science
Foundation of Shandong Province (ZR2015HL043),
and Shandong Science and Technology Program
(No.2014GSF118106) to ZX.
第一作者机构：[1]Department of Neurology, Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000 P.R. China
通讯作者：
推荐引用方式(GB/T 7714)：
Zhangyong Xia,Mingliang Gu,Xiaodong Jia,et al.Integrated DNA methylation and gene expression analysis identifies SLAMF7 as a key regulator of atherosclerosis[J].AGING-US.2018,10(6):1324-1337.doi:10.18632/aging.101470.
APA：
Zhangyong Xia,Mingliang Gu,Xiaodong Jia,Xiaoting Wang,Chunxia Wu...&Jiyue Wang.(2018).Integrated DNA methylation and gene expression analysis identifies SLAMF7 as a key regulator of atherosclerosis.AGING-US,10,(6)
MLA：
Zhangyong Xia,et al."Integrated DNA methylation and gene expression analysis identifies SLAMF7 as a key regulator of atherosclerosis".AGING-US 10..6(2018):1324-1337
