机构：[1]College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China[2]The Second Affiliated Hospital, Guangzhou University of Chinese Medicine, Guangzhou 510120, China广东省中医院[3]Sci-tech Industrial Park, Guangzhou University of Chinese Medicine, Guangzhou 510120, China
Two polysaccharides CAVAP-I and CAVAP-II from Citrus aurantium L. var. amara Engl showed significant structural differences. Average molecular weights of CAVAP-I and CAVAP-II were 10.5 kDa and 4.5 kDa. Triple-helix conformation existed in CAVAP-II rather than CAVAP-I. Arabinose, mannose, glucose and galactose formed main components of CAVAP-I and CAVAP-II at different ratios. (1 -> 3,6)-beta-D-Gal and (1 -> 5)-alpha-L-Ara composed the backbone of CAVAP-I and CAVAP-II. CAVAP-II displayed greater immune enhancement potential than CAVAP-I. CAVAP-II significantly promoted interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) production, and inducible nitric oxide synthase, IL-6, TNF-alpha and interleukin-1 beta mRNA expression by activating phosphorylated extracellular signal-regulated kinase, c-Jun N-terminal kinase, P38 and P65. These results indicated that structures were closely related to immunomodulatory activity of polysaccharides. CAVAP-II showed the greatest immunostimulation potential followed by CAVAP-I and CAVAPs by activating mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kappa B) pathways. (C) 2017 Elsevier Ltd. All rights reserved.
基金：
Science and Technology Project
of Guangzhou City (201604020150), Science and Technology Project
of Guangdong (2014A020212682), Special Research of Traditional
Chinese Medicine Hospital of Guangdong Province
(YN2014ZH02).
第一作者机构：[1]College of Food and Bioengineering, South China University of Technology, Guangzhou 510640, China
通讯作者：
推荐引用方式(GB/T 7714)：
Shen Chun-Yan,Jiang Jian-Guo,Li Ming-Qiang,et al.Structural characterization and immunomodulatory activity of novel polysaccharides from Citrus aurantium Linn. variant amara Engl[J].JOURNAL OF FUNCTIONAL FOODS.2017,35:352-362.doi:10.1016/j.jff.2017.05.055.
APA：
Shen, Chun-Yan,Jiang, Jian-Guo,Li, Ming-Qiang,Zheng, Chao-Yang&Zhu, Wei.(2017).Structural characterization and immunomodulatory activity of novel polysaccharides from Citrus aurantium Linn. variant amara Engl.JOURNAL OF FUNCTIONAL FOODS,35,
MLA：
Shen, Chun-Yan,et al."Structural characterization and immunomodulatory activity of novel polysaccharides from Citrus aurantium Linn. variant amara Engl".JOURNAL OF FUNCTIONAL FOODS 35.(2017):352-362
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