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Inhibition of the infectivity and inflammatory response of influenza virus by Arbidol hydrochloride in vitro and in vivo (mice and ferret)

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收录情况： ◇ SCIE

作者：
Wang, Yutao[1] * ; Ding, Yuewen[1,2] * ; Yang, Chunguang[1] * ; Li, Runfeng[1] ; Du, Qiuling[1] ; Hao, Yanbing[1] ; Li, Zhengtu[1] ; Jiang, Haiming[1] ; Zhao, Jin[1] ; Chen, Qiaoyan[1,3] ; Yang, Zifeng[1,4,*1] ; He, Zhanlong[5] ;
机构： [1]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, PR China [2]School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510515, PR China [3]Guangdong Provincial Hospital of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, 510006, PR China [4]Faculty of Chinese Medicine, Macau University of Science and Technology, Taipa, Macau SAR, PR China [5]Institute of Medical Biology, Chinese Academy of Medical Sciences, Peking Union medicine College, Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Diseases, Kunming, 650118, PR China
出处：
DOI： 10.1016/j.biopha.2017.04.091
ISSN：

关键词： Arbidol hydrochloride Influenza virus Anti-viral Anti-inflammatory

摘要：
Influenza virus infections are the main contagious respiratory disease with high levels of morbidity and mortality worldwide. Antiviral drugs are indispensable for the prophylaxis and treatment of influenza and other respiratory viral infections. In this study, the Arbidol hydrochloride (ARB), which has been licensed in Russia and China, is used to investigate its anti-viral and anti-inflammatory efficacy in vitro and in vivo. The antiviral results in vitro showed that ARB had a better inhibition on Influenza virus A/PR/8/34 (H1N1), A/Guangdong/GIRD07/09 (H1N1), A/Aichi/2/68 (H3N2), A/HK/Y280/97 (H9N2) with IC50 ranging from 4.4 to 12.1 mu M. The further mechanisms study demonstrated that ARB is able to inhibit hemagglutinin-mediated hemolysis at concentration of 3.91-15.63 mu g/mL. The anti-inflammatory efficacy in vitro indicated that IL-6, IP-10, MCP-1, RANTES and TNF-alpha levels were diminished by ARB at concentrations of 22.6 and 18.8 mu M. The in vivo results in mice model displayed that the survival rates of mice administered 25 mg/mL and 45 mg/mL ARB were 40% and 50% respectively. And also, ARB can inhibit the decrease of body weight at 45 mg/mL and inhibit the increase of mice lung index at 25 mg/mL and 45 mg/mL comparing to virus group. In ferret model, the ARB-treated ferrets showed a fever that peaked at 2 dpi and gradually decreased beginning at 3 dpi while relatively high temperatures were observed until 4 dpi in the virus group. The ARB-treated group scored 0-1 in the activity level at 2 dpi and 3 dpi at all time points. The transcription levels of cytokines in the respiratory tract of ferrets were detected at 3 dpi. Several proinflammatory cytokines induced by influenza (IL-10, TNF-alpha, IL-8 and IL-6) were down-regulated by post-treatment with ARB. The histopathological results of ferret lung displayed that ARB can alleviate the influenza virus induced lung lesions. Our results clarified the activity of ARB in both suppressing virus propagation and modulating the expression of inflammatory cytokines in vitro and in vivo, it can be as an effective drug to treat the influenza virus infection. (C) 2017 Elsevier Masson SAS. All rights reserved.

基金：
Shijiazhuang No. 4 Pharmaceutical Co., Ltd.; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81471937, U1301224]; Talent Training for Technological Innovation of Yunnan Province [2009CI117]; Applied Basic Research Program of Yunnan Province [2013FZ142]; Guangzhou City Board of education Science and Technology Programme [1201410111]; International S&T Coorperation Program of Guangdong Province [2013B051000085]; Engineering technology research center(development) of Guangdong general universities [GCZX-A1408]; Jiangsu Wuzhong Pharmaceutical China Co., Ltd.
语种：
外文
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中科院(CAS)分区：
出版当年[2016]版：
大类 | 3 区 医学
小类 | 4 区 医学：研究与实验 4 区 药学
最新[2025]版：
大类 | 2 区 医学
小类 | 2 区 医学：研究与实验 2 区 药学
JCR分区：
出版当年[2015]版：
Q3 MEDICINE, RESEARCH & EXPERIMENTAL Q3 PHARMACOLOGY & PHARMACY
最新[2023]版：
Q1 MEDICINE, RESEARCH & EXPERIMENTAL Q1 PHARMACOLOGY & PHARMACY

影响因子： 最新[2023版] 最新五年平均 出版当年[2015版] 出版当年五年平均 出版前一年[2014版] 出版后一年[2016版]

第一作者：
第一作者机构： [1]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, PR China
共同第一作者：
通讯作者：
通讯机构： [1]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, PR China [4]Faculty of Chinese Medicine, Macau University of Science and Technology, Taipa, Macau SAR, PR China [*1]State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510120, PR China.
推荐引用方式(GB/T 7714)：
Wang Yutao,Ding Yuewen,Yang Chunguang,et al.Inhibition of the infectivity and inflammatory response of influenza virus by Arbidol hydrochloride in vitro and in vivo (mice and ferret)[J].BIOMEDICINE & PHARMACOTHERAPY.2017,91:393-401.doi:10.1016/j.biopha.2017.04.091.
APA：
Wang, Yutao,Ding, Yuewen,Yang, Chunguang,Li, Runfeng,Du, Qiuling...&He, Zhanlong.(2017).Inhibition of the infectivity and inflammatory response of influenza virus by Arbidol hydrochloride in vitro and in vivo (mice and ferret).BIOMEDICINE & PHARMACOTHERAPY,91,
MLA：
Wang, Yutao,et al."Inhibition of the infectivity and inflammatory response of influenza virus by Arbidol hydrochloride in vitro and in vivo (mice and ferret)".BIOMEDICINE & PHARMACOTHERAPY 91.(2017):393-401

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