机构：[a]Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China[b]State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR,China[c]Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China[d]Institute Guangzhou of Advanced Technology, Chinese Academy of Sciences, Guangzhou, China[e]Department of ENT, Guangdong Province Traditional Chinese Medical Hospital, Guangzhou, China大德路总院耳鼻喉科广东省中医院
Ethnopharmacological relevance: Zao-Jiao-Ci (ZJC), as the spine of Chinese Honey locust (Gleditsia sinensis Lam.), is traditionally used as Chinese medicine to reduce inflammation. Aim of the study: The present study aimed to investigate an anti-inflammatory effect of ZJC aqueous extract both in vitro and in vivo, as well as its underlying mechanisms. Materials and methods: Anti-inflammatory effect of ZJC aqueous extract was evaluated by using carrageenan-induced paw edema in rats. In addition, the inhibitory effects of ZJC on nitric oxide production, intracellular reactive oxygen species production, pro-inflammatory mediator expression and prostaglandin E-2 (PGE(2)) production were determined by using LPS-activated RAW 264.7 cells. The anti-oxidant activity of ZJC was assessed using 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid assay. Results: ZJC aqueous extract showed significant suppressive effect on paw edema in rats at 100 mg/kg. Moreover, ZJC aqueous extract decreased the expression of cyclooxygenase (COX)-2 and significantly decreased the PGE(2), tumor necrosis factor-alpha, interleukin (IL)-1 beta and IL-6 production in LPS-activated macrophages in dose-dependent manners. ZJC aqueous extract inhibited the mRNA expression of these inflammatory cytokines as well. Furthermore, ZJC aqueous extract was found as an anti-oxidant and could inhibit ROS production in the LPS-induced cells. Conclusions: These findings show the potential of ZJC aqueous extract as a naturally occurring COX-2 inhibitor to reduce inflammation. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
基金：
State Key Laboratory of Phytochemistry and Plant Resources in West China (CUHK) from HKSAR; CUHKChinese University of Hong Kong; Chinese University of Hong KongChinese University of Hong Kong
第一作者机构：[a]Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China[b]State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR,China[c]Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China
通讯作者：
通讯机构：[a]Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China[b]State Key Laboratory of Phytochemistry and Plant Resources in West China, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR,China[c]Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China[e]Department of ENT, Guangdong Province Traditional Chinese Medical Hospital, Guangzhou, China[*1]3/F, Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
推荐引用方式(GB/T 7714)：
Kai KaiLi,XuelinZhou,HingLokWong,et al.In vivo and in vitro anti-inflammatory effects of Zao-Jiao-Ci (the spine of Gleditsia sinensis Lam.) aqueous extract and its mechanisms of action[J].JOURNAL OF ETHNOPHARMACOLOGY.2016,192:192-200.doi:10.1016/j.jep.2016.07.020.
APA：
Kai KaiLi,XuelinZhou,HingLokWong,ChunFaiNg,WeiMingFu...&ChunHayKo.(2016).In vivo and in vitro anti-inflammatory effects of Zao-Jiao-Ci (the spine of Gleditsia sinensis Lam.) aqueous extract and its mechanisms of action.JOURNAL OF ETHNOPHARMACOLOGY,192,
MLA：
Kai KaiLi,et al."In vivo and in vitro anti-inflammatory effects of Zao-Jiao-Ci (the spine of Gleditsia sinensis Lam.) aqueous extract and its mechanisms of action".JOURNAL OF ETHNOPHARMACOLOGY 192.(2016):192-200
