Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats
机构：[1]Department of Anatomy, Zhong Shan School of Medicine, Sun Yat‑sen University, Guangzhou, Guangdong 510080[2]Department of Radiology, The Fifth Affiliated Hospital of Sun Yat‑sen University, Zhuhai, Guangdong 519000[3]Department of Radiology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120[4]Department of Anesthesiology, The First Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510080[5]Department of Encephalopathy Center, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou,Guangdong 510120, P.R. China大德路总院脑病科广东省中医院
Although studies have been undertaken on gadolinium labeling-based molecular imaging in magnetic resonance imaging (MRI), the use of non-ionic gadolinium in the tracking of stem cells remains uncommon. To investigate the efficiency in tracking of stem cells with non-ionic gadolinium as an MRI contrast agent, a rhodamine-conjugated fluorescent reagent was used to label bone marrow stromal cells (BMSCs) of neonatal rats in vitro, and MRI scanning was undertaken. The fluorescent-conjugated cell uptake reagents were able to deliver gadodiamide into BMSCs, and cell uptake was verified using flow cytometry. In addition, the labeled stem cells with paramagnetic contrast medium remained detectable by an MRI monitor for a minimum of 28 days. The present study suggested that this method can be applied efficiently and safely for the labeling and tracking of bone marrow stromal cells in neonatal rats.
基金：
National Natural Science Foundation of China (grant nos. 81070995 and 31171290); the Doctoral Research Foundation of the Ministry of Education, China (grant no. 20110171110048); the Natural Science Foundation of Guangdong Province (grant no. S2012010009215); and the Health Bureau Foundation of Zhuhai City (grant no. 2014108).
第一作者机构：[1]Department of Anatomy, Zhong Shan School of Medicine, Sun Yat‑sen University, Guangzhou, Guangdong 510080[2]Department of Radiology, The Fifth Affiliated Hospital of Sun Yat‑sen University, Zhuhai, Guangdong 519000
通讯作者：
通讯机构：[1]Department of Anatomy, Zhong Shan School of Medicine, Sun Yat‑sen University, Guangzhou, Guangdong 510080[2]Department of Radiology, The Fifth Affiliated Hospital of Sun Yat‑sen University, Zhuhai, Guangdong 519000[*1]Department of Radiology, The Fifth Affiliated Hospital of Sun Yat-sen University, 52 Mei Hua East Road, Zhuhai, Guangdong 519000, P.R. China[*2]Department of Anatomy, Zhong Shan School of Medicine, Sun Yat-sen University, 74 Zhong Shan 2nd Road, Guangdong 510080, P.R. China
推荐引用方式(GB/T 7714)：
Li Ying-Qin,Tang Ying,Fu Rao,et al.Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats[J].MOLECULAR MEDICINE REPORTS.2015,12(1):913-920.doi:10.3892/mmr.2015.3532.
APA：
Li, Ying-Qin,Tang, Ying,Fu, Rao,Meng, Qiu-Hua,Zhou, Xue...&Zhou, Li-Hua.(2015).Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats.MOLECULAR MEDICINE REPORTS,12,(1)
MLA：
Li, Ying-Qin,et al."Efficient labeling in vitro with non-ionic gadolinium magnetic resonance imaging contrast agent and fluorescent transfection agent in bone marrow stromal cells of neonatal rats".MOLECULAR MEDICINE REPORTS 12..1(2015):913-920
