机构：[1]Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China[2]Department of Breast Surgery, Guangdong Traditional Chinese Medicine Hospital, Guangzhou 510000, Guangdong, China大德路总院乳腺科大德路总院乳腺科广东省中医院[3]Department of Plastic Surgery, The Third Affiliated Hospital of Guangzhou Medical College, Guangzhou 510150, Guangdong, China[4]Department of Pathology, Guangdong Traditional Chinese Medicine Hospital, Guangzhou 510000, Guangdong, China大德路总院珠海院区病理科病理科大德路总院病理科广东省中医院
This study aims to determine the relationship between Duffy antigen receptor for chemokines (DARC) and keloid pathogenesis. DARC expression was determined by immunohistochemistry, real-time PCR, and Western blot analysis. Cell proliferation was assessed by CCK-8 assay. Cell migration and invasion abilities were measured by the shift assay. Levels of CC chemokine ligand 2 (CCL2), CXC chemokine ligand 8 (CXCL8), and matrix metalloproteinase 2 (MMP2) were detected by real-time PCR and ELISA. Our results showed that DARC levels were elevated in human keloid fibroblasts. After knocking down DARC, cell proliferation was not altered, whereas the migration and invasion abilities of keloid fibroblasts were significantly elevated. Additionally, the mRNA expression levels of CCL2, CXCL8, and MMP2 were not influenced by DARC knockdown. However, the secretion of CCL2, but not CXCL8 or MMP2, was significantly increased after DARC knockdown. Our results suggest that DARC might inhibit the secretion of CCL2. Moreover, DARC knockdown increases the migration and invasion abilities of keloid fibroblasts. (C) 2015 Elsevier B.V. All rights reserved.
基金：
National Natural
Science Foundation of China (No. 81071589).
第一作者机构：[1]Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China[2]Department of Breast Surgery, Guangdong Traditional Chinese Medicine Hospital, Guangzhou 510000, Guangdong, China
通讯作者：
通讯机构：[1]Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, China[*1]Department of Plastic and Reconstructive Surgery, Nanfang Hospital, Southern Medical University, No. 1838, Guangzhou North Road, Guangzhou 510515, Guangdong, China.
推荐引用方式(GB/T 7714)：
Chen Ying,Liao Nong,Lu Feng,et al.The role of Duffy antigen receptor for chemokines in keloids[J].GENE.2015,570(1):44-49.doi:10.1016/j.gene.2015.05.071.
APA：
Chen, Ying,Liao, Nong,Lu, Feng,Peng, Hui&Gao, Jianhua.(2015).The role of Duffy antigen receptor for chemokines in keloids.GENE,570,(1)
MLA：
Chen, Ying,et al."The role of Duffy antigen receptor for chemokines in keloids".GENE 570..1(2015):44-49
