机构：[1]Institute of Tropical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006[2]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease,Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University,Guangzhou, Guangdong 510120[3]Key Laboratory of Livestock Disease Prevention of Guangdong Province,Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640, P.R. China[4]Centre of Immunology of Marseille‑Luminy, Aix‑Marseille University, 13009 Marseille, France
Influenza viruses often pose a serious threat to animals and human health. In an attempt to explore the potential of herbal medicine as a treatment for influenza virus infection, eleutheroside B1, a coumarin compound extracted from herba sarcandrae, was identified, which exhibited antiviral and anti‑inflammatory activities against influenza A virus. In this study, high‑throughput RNA sequencing and isobaric tags for relative and absolute quantification (iTRAQ) assays were performed to determine alterations in the non‑coding RNA (ncRNA) transcriptome and proteomics. Bioinformatics and target prediction analyses were used to decipher the potential roles of altered ncRNAs in the function of eleutheroside B1. Furthermore, long ncRNA (lncRNA) and mRNA co‑expressing networks were constructed to analyze the biological functions by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. The analysis of RNA sequencing data revealed that 5 differentially expressed ncRNAs were upregulated and 3 ncRNAs were downregulated in the A549 cells infected with A/PR8/34/H1N1, with or without eleutheroside B1 treatment (PR8+eleu and PR8, respectively). Nuclear paraspeckle assembly transcript 1 (NEAT1) was differentially expressed between the PR8 and A549 cell groups. GO and KEGG pathway analyses indicated that eleutheroside B1 took advantage of the host cell biological processes and molecular function for its antiviral and anti‑inflammatory activities, as well as for regulating cytokine‑cytokine receptor interaction in the immune system, consistent with previous findings. The results of the iTRAQ assays indicated that L antigen family member 3 (LAGE3) protein, essential for tRNA processing, tRNA metabolic processes and ncRNA processing, was downregulated in the PR8+eleu compared with the PR8 group. In the present study, these comprehensive, large‑scale data analysis enhanced the understanding of multiple aspects of the transcriptome and proteomics that are involved in the antiviral and anti‑inflammatory activities of eleutheroside B1. These findings demonstrate the potential of eleutheroside B1 for use in the prevention and treatment of influenza A virus‑mediated infections.
基金：
The present study was supported by the National Natural
Science Foundation of China (U1502226), the Engineering
technology research center (development) of Guangdong
general universities (GCZX‑A1408), Natural Science Basic
Research Program of Shaanxi (Program No. 2019JM‑513) and
Shijiazhuang Yiling Pharmaceutical Co.,Ltd (Shijiazhuang,
China).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|3 区医学
小类|4 区医学：研究与实验
最新[2025]版：
大类|2 区医学
小类|2 区医学：研究与实验
第一作者：
第一作者机构：[1]Institute of Tropical Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006[2]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease,Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University,Guangzhou, Guangdong 510120
共同第一作者：
通讯作者：
通讯机构：[2]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease,Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University,Guangzhou, Guangdong 510120[*1]State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, First Affiliated Hospital of Guangzhou Medical University, 195 Dongfengxi Road, Guangzhou, Guangdong 510120, P.R. China
推荐引用方式(GB/T 7714)：
Yan Wen,Chen Jing,Wei Zhenquan,et al.Effect of eleutheroside B1 on non‑coding RNAs and protein profiles of influenza A virus‑infected A549 cells.[J].International journal of molecular medicine.2020,45(3):753-768.doi:10.3892/ijmm.2020.4468.
APA：
Yan Wen,Chen Jing,Wei Zhenquan,Wang Xiaohu,Zeng Zhiqi...&Wang Xinhua.(2020).Effect of eleutheroside B1 on non‑coding RNAs and protein profiles of influenza A virus‑infected A549 cells..International journal of molecular medicine,45,(3)
MLA：
Yan Wen,et al."Effect of eleutheroside B1 on non‑coding RNAs and protein profiles of influenza A virus‑infected A549 cells.".International journal of molecular medicine 45..3(2020):753-768
