详情页 - 广东省中医院知识库

当前位置：首页 > 详情页

Construction of electrochemical aptasensor of carcinoembryonic antigen based on toehold-aided DNA recycling signal amplification.

文献详情

资源类型：

Pubmed体系：

收录情况： ◇ EI

作者：

机构： [a]Affiliated Dongfeng Hospital, Hubei University of Medicine, Shiyan 442008, Hubei, China [b]Department of General Surgery, Shenzhen Nanshan People’s, Shenzhen 518000, Guangdong, China [c]Shiyan Hospital of Integrated Traditional and Western Medicine, Shiyan 442008, Hubei, China [d]Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Hubei University of Medicine, Shiyan 442008, Hubei, China

出处：

DOI：

ISSN：

关键词： Electrochemical aptasensor Strand displacement reaction Carcinoembryonic antigen Methylene blue Cancer

摘要：

Carcinoembryonic antigen (CEA), serves as a broad-spectrum tumor marker, and plays an important role in reflecting the existence, therapeutic evaluation, development, monitoring and prognosis of many types of cancer. An electrochemical aptasensor was designed for CEA detection based on toehold-aided DNA recycling. A partially hybridized Probe-4 (i.e. P2/P3/P4) was self-assembled on the surface of a gold electrode serving as the sensing platform. For CEA detection, CEA can bind with aptamer and free probe-1 (P1) can hybridize with P4, triggering toehold-aided DNA recycling. This enables the hybridization of more probe-5 (P5) (labeled with methylene blue (MB)) with P4, causing more methylene blue (MB) to be brought close to the electrode surface. An amplified current signal was thus generated due to more MB in the electrode surface. The proposed design showed good linearity between current response and log CEA concentration ranging from 0.1 to 50 ng·mL-1, with a detection limit of 20 pg mL-1. This aptasensor also showed high specificity for CEA detection, and was successfully used in spiked biological samples. Copyright © 2020 Elsevier B.V. All rights reserved.

基金：

语种：

PubmedID：

中科院(CAS)分区：

出版当年[2019]版：

大类 | 2 区化学

小类 | 2 区生物学 2 区生物物理 3 区生化与分子生物学 3 区电化学

最新[2025]版：

大类 | 2 区化学

小类 | 2 区生物学 2 区生物物理 2 区电化学 3 区生化与分子生物学

第一作者：

第一作者机构： [a]Affiliated Dongfeng Hospital, Hubei University of Medicine, Shiyan 442008, Hubei, China

共同第一作者：

通讯作者：

通讯机构： [a]Affiliated Dongfeng Hospital, Hubei University of Medicine, Shiyan 442008, Hubei, China [d]Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Hubei University of Medicine, Shiyan 442008, Hubei, China [*1]Affiliated Dongfeng Hospital, Hubei University of Medicine, Shiyan 442008, Hubei, China

推荐引用方式(GB/T 7714)：

APA：

MLA：

相关文献

[1]A highly sensitive electrochemical aptasensor for vascular endothelial growth factor detection based on toehold-mediated strand displacement reaction. [2]Sentinel lymph node detection using methylene blue in patients with early stage cervical cancer [3]Simultaneous quantitation of carbohydrate antigen 125 and carcinoembryonic antigen in human serum via time-resolved fluoroimmunoassay [4]Aggressive variant prostate cancer: A case report and literature review [5]3种肿瘤标志物检测在乳腺癌术后随访中的价值 [6]亚甲蓝在继发性甲旁亢手术中对甲状旁腺的定位作用 [7]Reagentless and sensitive determination of carcinoembryonic antigen based on a stable Prussian blue modified electrode. [8]血清NSE、SCC、CYFRA21-1和CEA联合检测对肺癌的诊断价值 [9]Electrochemical aptasensor based on proximity binding-induced DNA networked for enzyme-free and ultrasensitive detection of thrombin [10]A stepwise mutagenesis approach using histidine and acidic amino acid to engineer highly pH-dependent protein switches.