机构：[a]Institute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, 510632, China.[b]The First School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, China.[c]Guangzhou University of Chinese Medicine, Guangzhou, 510010, China[d]Guangdong Key Lab of Orthopedic Technology and Implant, General Hospital of Southern Theater Command of PLA, 111 Liuhua Road, Guangzhou, 510010, China
The strontium-substituted hydroxyapatite (SrHA) is a commonly used material in bone regeneration for its good osteoconductivity and high alkaline phosphatase (ALP) activity. Scaffolds used in bone defects require a high compressive modulus. However, the SrHA nanoparticle-doped scaffold cannot properly fit the required mechanical properties. Therefore, a lot of effort has been used to fabricate synthetic bone scaffolds with biocompatibility, suitable mechanical properties, antibacterial ability and osteoconductivity. Here, we used a facile hydrothermal method to synthesize graphene oxide (GO)-reinforced SrHA nanoparticles. The incorporation of GO can be used as nucleation and growth active sites of hydroxyapatite. In addition, GO is easy to self-assemble into a layered structure in the dispersion, which can effectively regulate the deposition of hydroxyapatite on the surface of GO. The scaffold was fabricated using a freeze-drying method by incorporating SrHA/GO nanoparticles into chitosan (CS) and quaternized chitosan (QCS) mixed solutions. The compressive modulus of the CS/QCS/SrHA/GO scaffold reached 438.5 kPa, which was 4-fold higher than that of the CS/QCS scaffold. The CS/QCS/SrHA/GO scaffold exhibited significantly higher in vitro mineralization levels and ALP activity. In vivo rat skull repair indicated that the CS/QCS/SrHA/GO scaffold had a significant role in promoting bone regeneration. This study provides a new strategy for modifying hydroxyapatite to satisfy the biomedical demand of bone tissue engineering scaffolds.
基金：
This study was supported by Key Laboratory of Trauma & Tissue Repair of Tropical
Area of PLA, the National Natural Science Foundation of China (81601884,
81972080), the Research Program of PLA (CGZ16C004), the Science and
Technology Planning Project of Guangdong Province (2017B030314139,
2014A020215025), the Natural Science Foundation of Guangdong Province
(2015A030312004), Medical research foundation of Guangdong province
(A2019228) and the Postdoctoral Fund of the First Affiliated Hospital, Jinan
University (801313).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|2 区工程技术
小类|2 区材料科学：生物材料
最新[2025]版：
大类|3 区医学
小类|3 区材料科学：生物材料
第一作者：
第一作者机构：[a]Institute of Orthopedic Diseases and Center for Joint Surgery and Sports Medicine, The First Affiliated Hospital, Jinan University, Guangzhou, 510632, China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Tingting Wu,Binglin Li,Wanshun Wang,et al.Strontium-substituted hydroxyapatite grown on graphene oxide nanosheet-reinforced chitosan scaffold to promote bone regeneration.[J].Biomaterials science.2020,doi:10.1039/d0bm00523a.
APA：
Tingting Wu,Binglin Li,Wanshun Wang,Lingling Chen,Zhan Li...&Tao Zhang.(2020).Strontium-substituted hydroxyapatite grown on graphene oxide nanosheet-reinforced chitosan scaffold to promote bone regeneration..Biomaterials science,,
MLA：
Tingting Wu,et al."Strontium-substituted hydroxyapatite grown on graphene oxide nanosheet-reinforced chitosan scaffold to promote bone regeneration.".Biomaterials science .(2020)
