机构：[1]Department of Traumatology, The Third Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, 22 Jiangnan‑Xi Road, Guangzhou 510240, Guangdong, People’s Republic of China.深圳市中医院深圳医学信息中心[2]Key Laboratory of Orthopaedics & Traumatology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510000, Guangdong, People’s Republic of China.[3]Department of Diagnostics of Chinese Medicine, School of The Basic Medicine, Guangzhou 510006, Guangdong, People’s Republic of China.
Bushen Huoxue decoction (BHD) has a significant effect on fracture rehabilitation, yet its underlying mechanism is still unknown. The purpose of this study was to explore whether BHD promotes bone marrow mesenchymal stem cell (BMSC) migration through the Wnt5a signalling pathway.
BHD was extracted by petroleum, and its composition was analysed. Cell viability in the presence of various concentrations of BHD for 24, 48 and 72 h was measured using a Cell Counting Kit-8 assay. Transwell assays and wound healing assays were used to observe the migration ability of BMSCs. Lentiviral vectors were used to knock down Wnt5a. Polymerase chain reaction and Western blot analyses were used to further compare Wnt5a signalling components at the mRNA and protein levels between groups.
BHD treatment groups showed increased migration ability and Wnt5a expression. Knocking down Wnt5a using a lentivirus significantly inhibited the effects of BHD, which implies that BHD promotes BMSC migration ability through activation of Wnt5a.
BHD can enhance BMSC migration, possibly by activating Wnt5a signalling.
基金：
This work was supported by the National Natural Science Foundation of China
(Nos. 81473699, 81503593, 81273783) and the Natural Science Foundation of
Guangdong Province (CN) (2016A030313649, 2016A030310289).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|4 区医学
小类|3 区全科医学与补充医学4 区药学
最新[2025]版：
大类|2 区医学
小类|2 区全科医学与补充医学2 区药学
第一作者：
第一作者机构：[1]Department of Traumatology, The Third Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, 22 Jiangnan‑Xi Road, Guangzhou 510240, Guangdong, People’s Republic of China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Wei Shen,Hui Luo,Liangliang Xu,et al.Wnt5a mediates the effects of Bushen Huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro.[J].Chinese medicine.2018,13:45.doi:10.1186/s13020-018-0200-2.
APA：
Wei Shen,Hui Luo,Liangliang Xu,Zhifang Wu,Hongtai Chen...&Yiwen Luo.(2018).Wnt5a mediates the effects of Bushen Huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro..Chinese medicine,13,
MLA：
Wei Shen,et al."Wnt5a mediates the effects of Bushen Huoxue decoction on the migration of bone marrow mesenchymal stem cells in vitro.".Chinese medicine 13.(2018):45
