机构：[1]College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510632, P.R. China.[2]Department of Diagnostics, Zhuhai Campus of Zunyi Medical University, Zhuhai, Guangdong 519041, P.R. China.[3]School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming, Yunnan 650500, P.R. China.[4]Department of Pharmaceutical Sciences, Zhuhai Campus of Zunyi Medical University, Zhuhai, Guangdong 519041, P.R. China.[5]Department of Gastroenterology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong 519000, P.R. China.
Gardenia fruit has been used in traditional Chinese medicine for thousands of years. A previous study by the present authors indicated that the ethanol extract of gardenia fruits (EEG) primarily contains eight constituents. In the present study, the potential effects of EEG on unilateral ureteral obstruction (UUO)-induced renal interstitial fibrosis were observed in rats. A total of 30 rats were randomly divided into three groups (n=10 each): Sham group, UUO group, and EEG group, which were administered with EEG (200 mg/kg/day) or the same volume of distilled water as a vehicle. UUO were established by ligating left ureter at two points and cut between the ligatures. All rats were sacrificed at 14 days after UUO operation. the present results demonstrated that EEG significantly elevated the expressions of vascular endothelial growth factor and E-cadherin induced by UUO (both P<0.05), and reduced levels of hypoxia-inducible factor-1α, transforming growth factor-β1, connective tissue growth factor and α-smooth muscle actin (all P<0.05). The present findings suggest that EEG is a potential novel renoprotective compound for renal fibrosis through inhibiting epithelial-to-mesenchymal transition.
基金：
Science and Technology Department of Guizhou Province [grant no. J(2010)2], the Project Natural Science Foundation of China (grant no. 21562051) and the project of Science and Technology Agency of Guizhou Province [grant no. J(2015) 2157].
第一作者机构：[1]College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510632, P.R. China.[2]Department of Diagnostics, Zhuhai Campus of Zunyi Medical University, Zhuhai, Guangdong 519041, P.R. China.
通讯作者：
通讯机构：[4]Department of Pharmaceutical Sciences, Zhuhai Campus of Zunyi Medical University, Zhuhai, Guangdong 519041, P.R. China.[*1]Department of Pharmaceutical Sciences, Zhuhai Campus of Zunyi Medical University, 368 Jinwan Road, Zhuhai, Guangdong 519041, P.R. China
推荐引用方式(GB/T 7714)：
Li Xiaobo,Ma Min,Zhang Xianggui,et al.Ethanol extract of gardenia fruit alleviates renal interstitial fibrosis induced by unilateral ureteral obstruction in rats.[J].EXPERIMENTAL AND THERAPEUTIC MEDICINE.2017,14(2):1381-1388.doi:10.3892/etm.2017.4662.
APA：
Li Xiaobo,Ma Min,Zhang Xianggui,Deng Liang,Wang Yarong...&Chen Yang.(2017).Ethanol extract of gardenia fruit alleviates renal interstitial fibrosis induced by unilateral ureteral obstruction in rats..EXPERIMENTAL AND THERAPEUTIC MEDICINE,14,(2)
MLA：
Li Xiaobo,et al."Ethanol extract of gardenia fruit alleviates renal interstitial fibrosis induced by unilateral ureteral obstruction in rats.".EXPERIMENTAL AND THERAPEUTIC MEDICINE 14..2(2017):1381-1388
