机构：[1]Key Laboratory of Molecular and Clinical Pharmacology, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, China[2]Infinitus (China) Company Ltd., Jiangmen 529156, China[3]Pediatric Department, Guangdong Women and Children Hospital and Health Institute, Guangzhou 511400, China[4]School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China
Natural products from the genus Euphorbia show attention-attracting activities, such as anticancer activity. In this article, classical isolation and structure identification were used in a study on Caper Euphorbia Seed. Subsequently, MTT and wound healing assays, flow cytometry, western blotting, Hoechst 33258 staining and fluorescence microscopy examination were applied to investigate the anticancer activity of the obtained compounds. In a result, lathyrol-3-phenyl- acetate-5,15-diacetate (deoxy Euphorbia factor L1, DEFL1) was isolated from Caper Euphorbia Seed. Moreover, the NMR signals were totally assigned. DEFL1 showed potent inhibition against lung cancer A549 cells, with an IC50 value of 17.51 ± 0.85 μM. Furthermore, DEFL1 suppressed wound healing of A549 cells in a concentration-dependent manner. Mechanically, DEFL1 induced apoptosis, with involvement of an increase of reactive oxygen species (ROS), decrease of mitochondrial membrane potential (ΔΨm), release of cytochrome c, activity raise of caspase-9 and 3. Characteristic features of apoptosis were observed by fluorescence microscopy. In summary, DEFL1 inhibited growth and induced apoptosis in lung cancer A549 cells via a mitochondrial pathway.
基金：
National Natural Science Foundation of China (81473320),
Fund of Guangdong Science and Technology Department (2016A020226024), Fund of Guangdong Education
Department (2015KTSCX112), Fund of Guangzhou Science and Technology Program (201707010048), Fund of
Traditional Chinese Medicine Bureau of Guangdong Province (20161181) and Research Fund of undergraduates
from Guangzhou Medical University
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|3 区化学
小类|3 区有机化学
最新[2025]版：
大类|3 区化学
小类|3 区生化与分子生物学3 区化学：综合
第一作者：
第一作者机构：[1]Key Laboratory of Molecular and Clinical Pharmacology, School of Pharmaceutical Sciences and the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, China
通讯作者：
推荐引用方式(GB/T 7714)：
Jian-ye Zhang,Wen-jing Huang,Hong-mei Sun,et al.Structure Identification and In Vitro Anticancer Activity of Lathyrol-3-phenylacetate-5,15-diacetate.[J].Molecules (Basel, Switzerland).2017,22(9):doi:10.3390/molecules22091412.
APA：
Jian-ye Zhang,Wen-jing Huang,Hong-mei Sun,Yun Liu,Xiao-qin Zhao...&Hu-biao Chen.(2017).Structure Identification and In Vitro Anticancer Activity of Lathyrol-3-phenylacetate-5,15-diacetate..Molecules (Basel, Switzerland),22,(9)
MLA：
Jian-ye Zhang,et al."Structure Identification and In Vitro Anticancer Activity of Lathyrol-3-phenylacetate-5,15-diacetate.".Molecules (Basel, Switzerland) 22..9(2017)
