机构：[1]Department of Obstetrics and Gynecology, Maternity and Children’s Healthcare Hospital of Foshan, Foshan 528000Guangdong, China.[2]Department of Obstetrics and Gynecology, Guangzhou First People’s Hospital, Guangzhou Medical University, Guangzhou 510180, China. 3Foshan Hospital of TCM, Foushan 52800, China.
Ovarian cancer is a possibly lethal gynecological malignancy and this study utilized phage display technology to screen and identify peptides that specifically bind to ovarian cancer cells and explored the effects of these peptides on ovarian cancer cells in vitro and in vivo.
The phage displayed peptide library was used to isolate the peptides binding to and internalizing into the ovarian carcinoma cells. Positive phage clones were characterized with DNA sequencing and bioinformatics analysis and then validated with immunofluorescence. Subsequently, the selected peptides were investigated for their cancer-related functions, including cell adhesion, spreading, motility, and invasion in vitro and in vivo.
Peptide1 read as SWQIGGNwas the positive peptide and showed preferential binding to the target cells. Peptide 1 also inhibited cell proliferation, migration, invasion and adhesion of ovarian cancer HO8910 cells in vitro. In vivo, Peptide 1 led to a lower tumorigenicity of HO8910 cells, which was characterized by the inhibitory effect on tumor growth and metastasis of ovarian cells.
These studies demonstrate that the phage display-identified tumor cell-binding peptide was able to control ovarian cancer cell viability, migration, invasion, and adhesion capacity in vitro as well as tumor growth and metastasis in vivo. Future studies will be aimed at evaluating the clinical efficacy of the peptide SWQIGGN in ovarian cancer patients.
基金：
Natural Science Foundation of Guangdong Province, China (#S2012010009341) and Bureau of Science
and Technology of Guangzhou Municipality, China (#11A53150713).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2014]版：
大类|3 区医学
小类|3 区肿瘤学
最新[2025]版：
大类|3 区医学
小类|3 区肿瘤学
第一作者：
第一作者机构：[1]Department of Obstetrics and Gynecology, Maternity and Children’s Healthcare Hospital of Foshan, Foshan 528000Guangdong, China.
通讯作者：
推荐引用方式(GB/T 7714)：
Zhou Cong,Kang Jiali,Wang Xiaoxia,et al.Phage display screening identifies a novel peptide to suppress ovarian cancer cells in vitro and in vivo in mouse models.[J].BMC cancer.2015,15:889.doi:10.1186/s12885-015-1891-8.
APA：
Zhou Cong,Kang Jiali,Wang Xiaoxia,Wei Wei&Jiang Wenyan.(2015).Phage display screening identifies a novel peptide to suppress ovarian cancer cells in vitro and in vivo in mouse models..BMC cancer,15,
MLA：
Zhou Cong,et al."Phage display screening identifies a novel peptide to suppress ovarian cancer cells in vitro and in vivo in mouse models.".BMC cancer 15.(2015):889
