机构：[1]The Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China[2]Cancer Center, Traditional Chinese Medicine Integrated Hospital, Southern Medical University, Guangzhou, Guangdong, China[3]Department of Medical Oncology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China浙江大学医学院附属第一医院
Heparanase(HPSE), an endo-beta-D-glucuronidase, is found overexpressed in Ovarian cancer (OC). The purpose of our work was to investigate primitively the possible role of HPSE in the development of OC. In this study, RNA interference (RNAi) with a HPSE small hairpin RNAs(HPSE-shRNA) and plasmid with HPSE were used to identify the effects of HPSE on the regulation of malignant behaviors of OC. OV-90 and SKOV3 were selected as a cell model in vitro and in vivo. The results showed that down-regulation of HPSE can significantly inhibit the proliferative and invasive ability of SKOV3 cells, and upregulation of HPSE in OV-90 cells showed the opposite effects. Compared with the parental OC cells, HPSE silencing cells exhibited attenuated capacities in developing tumor in nude mice, while the growth tumors xenografts derived from these cells were dramatically regressed. In conclusion, our results suggest that HPSE contributes to the proliferation and metastasis of OC and HPSE might be a potent molecular target for OC treatment.
基金：
Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Guangdong Province [S2013040013 849]; Science & Technology Project of Guangzhou (Democratic Science and Technology Major Project) [2012Y2-00022]; School Foundation of Guangzhou Medical University [2012C54]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81401874]
第一作者机构：[1]The Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China[*1]The Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510315, China
共同第一作者：
通讯作者：
通讯机构：[1]The Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China[*1]The Institute of Gynecology and Obstetrics, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510315, China
推荐引用方式(GB/T 7714)：
Zheng Haiyan,Ruan Jian,Zhao Peng,et al.Heparanase is involved in proliferation and invasion of ovarian cancer cells[J].CANCER BIOMARKERS.2015,15(5):525-534.doi:10.3233/CBM-150459.
APA：
Zheng, Haiyan,Ruan, Jian,Zhao, Peng,Chen, Shiping,Pan, Linglan&Liu, Jianqiao.(2015).Heparanase is involved in proliferation and invasion of ovarian cancer cells.CANCER BIOMARKERS,15,(5)
MLA：
Zheng, Haiyan,et al."Heparanase is involved in proliferation and invasion of ovarian cancer cells".CANCER BIOMARKERS 15..5(2015):525-534
