机构：[a]Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China[b]Department of Scientific research, Southern Medical University, Guangzhou, Guangdong, China[c]Department of Rheumatology and Immunology, Affiliated Hospital of Luzhou Medical College, Luzhou, Sichuan, China[d]Lab of molecular pathogen, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, Guangdong, China
Sinomenine, a purified alkaloid extracted from Sinomenium acutum, has been employed to treat arthritis for a long time. Previous studies have shown that sinomenine has favorable function of immunosuppression, but the exact mechanism has not been illustrated yet. In this study, we investigated the effect of sinomenine on human monocyte-derived dendritic cells (DCs). Lipopolysaccharide (LPS) induced DCs, exposed to sinomenine or only medium, were tested for their maturation parameters, cytokine expression, mixed lymphocyte reaction, activity and translocation of nuclear factor-kappa B (NF-kappaB) and phosphorylation of p38 stress-activated protein kinase (p38SAPK). Down-regulated membrane expression of CD40, CD80, CD83, CD86, and HLA-DR were found on DCs exposed to sinomenine. Similar result was noticed in their IL-12 production. Reduced activity and translocation of NF-kappaB was also detected in sinomenine treated DCs, except for the level of p38SAPK phosphorylation. Our results support the conclusion that sinomenine inhibits immune responses through suppressing the functions of antigen-presenting cells and NF-kappaB pathway is involved in their maturation cascade and T-cell activation. The findings indicate the potency for sinomenine to be generally used in DCs-mediated autoimmune diseases.
Sinomenine, a purified alkaloid extracted from Sinomenium acutum, has been employed to treat arthritis for a long time.
Previous studies have shown that sinomenine has favorable function of immunosuppression, but the exact mechanism has not been
illustrated yet. In this study, we investigated the effect of sinomenine on human monocyte-derived dendritic cells (DCs).
Lipopolysaccharide (LPS) induced DCs, exposed to sinomenine or only medium, were tested for their maturation parameters,
cytokine expression, mixed lymphocyte reaction, activity and translocation of nuclear factor-kappa B (NF-κB) and phosphorylation
of p38 stress-activated protein kinase (p38SAPK). Down-regulated membrane expression of CD40, CD80, CD83, CD86, and
HLA-DR were found on DCs exposed to sinomenine. Similar result was noticed in their IL-12 production. Reduced activity and
translocation of NF-κB was also detected in sinomenine treated DCs, except for the level of p38SAPK phosphorylation. Our results
support the conclusion that sinomenine inhibits immune responses through suppressing the functions of antigen-presenting cells
and NF-κB pathway is involved in their maturation cascade and T-cell activation. The findings indicate the potency for sinomenine
to be generally used in DCs-mediated autoimmune diseases.
基金：
This work is supported by National Science Foundation
of China (30400614).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2006]版：
大类|3 区医学
最新[2025]版：
大类|2 区医学
小类|2 区药学3 区免疫学
第一作者：
第一作者机构：[a]Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China
通讯作者：
推荐引用方式(GB/T 7714)：
Yi Zhao,Juan Li,Keqiang Yu,et al.Sinomenine inhibits maturation of monocyte-derived dendritic cells through blocking activation of NF-kappa B.[J].International immunopharmacology.2007,7(5):637-45.doi:10.1016/j.intimp.2007.01.007.
APA：
Yi Zhao,Juan Li,Keqiang Yu,Yi Liu&Xiaoguang Chen.(2007).Sinomenine inhibits maturation of monocyte-derived dendritic cells through blocking activation of NF-kappa B..International immunopharmacology,7,(5)
MLA：
Yi Zhao,et al."Sinomenine inhibits maturation of monocyte-derived dendritic cells through blocking activation of NF-kappa B.".International immunopharmacology 7..5(2007):637-45
