机构：[1]Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin 130062[2]College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin 130118[3]The Second Clinical School of Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangdong Provincial Hospital of Chinese Medicine‑Zhuhai Hospital, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510120, P.R. China珠海院区广东省中医院
Eupafolin is a flavonoid extracted from common sage. Previous studies have reported that Eupafolin has antioxidant, anti-inflammatory, and anti-tumor effects. However, its role in breast cancer remains unclear. The present study investigated the effects and underlying mechanism of action of Eupafolin using breast cancer cell lines. The effects of Eupafolin on breast cancer cell proliferation, migration, apoptosis and the cell cycle were determined. Cell viability and Transwell assays, reverse transcription-quantitative PCR, flow cytometry and western blot analysis were used in this study. The data showed that the proliferation, migration and invasion ability of EO771 cells treated with Eupafolin was significantly decreased, and the apoptosis rate was increased compared with that of the control. The protein levels of Bax and cleaved caspase 3 increased, whereas that of Bcl-2 decreased. In addition, Eupafolin treatment also caused the proliferation of breast cancer cells to be arrested at the G(0)/G(1) phase. Furthermore, results from western blotting indicated that Eupafolin treatment decreased the protein levels of p-PI3K, p-Akt and p-mTOR. Taken together, the present findings demonstrate that Eupafolin has a significant inhibitory effect on the proliferation of EO771 cells, inhibits cell migration and invasion, and promotes cell apoptosis, thereby causing G(0)/G(1) phase arrest, at least partially through the PI3K/Akt/mTOR signaling pathway. Therefore, the findings provide novel insights regarding the use of Eupafolin for the treatment of breast cancer.
基金：
Jilin Province Science and Technology Development Project [20200703014ZP]
第一作者机构：[1]Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin 130062
共同第一作者：
通讯作者：
通讯机构：[1]Department of Laboratory Animals, College of Animal Sciences, Jilin University, Changchun, Jilin 130062[*1]Department of Laboratory Animals, College of Animal Sciences, Jilin University, 5333 Xi’an Road, Changchun, Jilin 130062, P.R. China
推荐引用方式(GB/T 7714)：
Wei Jiahui,Zhang Xuefeng,Pan Huihao,et al.Eupafolin inhibits breast cancer cell proliferation and induces apoptosis by inhibiting the PI3K/Akt/mTOR pathway[J].ONCOLOGY LETTERS.2021,21(4):doi:10.3892/ol.2021.12593.
APA：
Wei, Jiahui,Zhang, Xuefeng,Pan, Huihao,He, Song,Yuan, Bao...&Ding, Yu.(2021).Eupafolin inhibits breast cancer cell proliferation and induces apoptosis by inhibiting the PI3K/Akt/mTOR pathway.ONCOLOGY LETTERS,21,(4)
MLA：
Wei, Jiahui,et al."Eupafolin inhibits breast cancer cell proliferation and induces apoptosis by inhibiting the PI3K/Akt/mTOR pathway".ONCOLOGY LETTERS 21..4(2021)
