Identification of IL-6 as a potential mediator of the myocardial fibrosis that occurs in response to surgery with cardiopulmonary bypass in children with Tetralogy of Fallot.
机构：[1]Department of Anesthesiology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, China[2]Basic Nursing Teaching and Research Section, Guangxi School of Traditional Chinese Medicine, Nanning, Guangxi, China[3]Department of Anesthesiology, Liuzhou Hospital of Traditional Chinese Medicine, Liuzhou, Guangxi, China[4]Department of Anesthesiology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China
Tetralogy of Fallot is a common CHD. Studies have shown a close link between heart failure and myocardial fibrosis. Interleukin-6 has been suggested to be a post-independent factor of heart failure. This study aimed to explore the relationship between IL-6 and myocardial fibrosis during cardiopulmonary bypass.We downloaded the expression profile dataset GSE132176 from Gene Expression Omnibus. After normalising the raw data, Gene Set Enrichment Analysis and differential gene expression analysis were performed using R. Further, a weighted gene correlation network analysis and a protein-protein interaction network analysis were used to identify HUB genes. Finally, we downloaded single-cell expression data for HUB genes using PanglaoDB.There were 119 differentially expressed genes in right atrium tissues comparing the post-CPB group with the pre-CPB group. IL-6 was found to be significantly up-regulated in the post-CPB group. Six genes (JUN, FOS, ATF3, EGR1, IL-6, and PTGS2) were identified as HUB genes by a weighted gene correlation network analysis and a protein-protein interaction network analysis. Gene Set Enrichment Analysis showed that IL-6 affects the myocardium during CPB mainly through the JAK/STAT signalling pathway. Finally, we used PanglaoDB data to analyse the single-cell expression of the HUB genes.Our findings suggest that high expression of IL-6 and the activation of the JAK/STAT signalling pathway during CPB maybe the potential mechanism of myocardial fibrosis. We speculate that the high expression of IL-6 might be an important factor leading to heart failure after ToF surgery. We expect that these findings will provide a basis for the development of targeted drugs.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|4 区医学
小类|4 区心脏和心血管系统4 区儿科
最新[2025]版：
大类|4 区医学
小类|4 区心脏和心血管系统4 区儿科
第一作者：
第一作者机构：[1]Department of Anesthesiology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, China[*1]Department of Anesthesiology, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, China.
通讯作者：
通讯机构：[1]Department of Anesthesiology, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, China[4]Department of Anesthesiology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China[*1]Department of Anesthesiology, The First Affiliated Hospital of Jinan University, 510632, Guangzhou, Guangdong, China.[*2]Department of Anesthesiology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, China.
推荐引用方式(GB/T 7714)：
Hongtao Liu,Yun Zhang,Zhenhai Wu,et al.Identification of IL-6 as a potential mediator of the myocardial fibrosis that occurs in response to surgery with cardiopulmonary bypass in children with Tetralogy of Fallot.[J].Cardiology in the young.2021,1-7.doi:10.1017/S1047951121001803.
APA：
Hongtao Liu,Yun Zhang,Zhenhai Wu&Liangqing Zhang.(2021).Identification of IL-6 as a potential mediator of the myocardial fibrosis that occurs in response to surgery with cardiopulmonary bypass in children with Tetralogy of Fallot..Cardiology in the young,,
MLA：
Hongtao Liu,et al."Identification of IL-6 as a potential mediator of the myocardial fibrosis that occurs in response to surgery with cardiopulmonary bypass in children with Tetralogy of Fallot.".Cardiology in the young .(2021):1-7
