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Bioinformatics screening the novel and promising targets of curcumin in hepatocellular carcinoma chemotherapy and prognosis.

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机构: [1]Scientifc Research Center, The First Afliated Hospital of Guangdong Pharmaceutical University, Gonghexiheng Street 1, Guangzhou, Guangdong 510080, P.R. China [2]Department of Head and Neck Radiotherapy, Meizhou City People’s Hospital, No.6 Building, Huangtang Road 63, Meijiang District, Meizhou, Guangdong 514031, P.R. China [3]Department of Traditional Chinese Medicine, The First Afliated Hospital of Guangdong Pharmaceutical University, Gonghexiheng Street 1, Guangzhou, Guangdong 510080, P.R. China. [4]Key Specialty of Clinical Pharmacy, The First Afliated Hospital of Guangdong Pharmaceutical University, Nonglin Down Street 19, Guangzhou, Guangdong 510080, P.R. China
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关键词: Bioinformatics analysis Hepatocellular carcinoma Curcumin Chemotherapy Prognosis

摘要:
The aim of present study was to screen the novel and promising targets of curcumin in hepatocellular carcinoma diagnosis and chemotherapy.Potential targets of curcumin were screened from SwissTargetPrediction, ParmMapper and drugbank databases. Potential aberrant genes of hepatocellular carcinoma were screened from Genecards databases. Fifty paired hepatocellular carcinoma patients' gene expression profiles from the GEO database were used to test potential targets of curcumin. Besides, GO analysis, KEGG pathway enrichment analysis and PPI network construction were used to explore the underlying mechanism of candidate hub genes. ROC analysis and Kaplan-Meier analysis were used to evaluate the diagnostic and prognostic value of candidate hub genes, respectively. Real-time PCR was used to verify the results of bioinformatics analysis.Bioinformatics analysis results suggested that AURKA, CDK1, CCNB1, TOP2A, CYP2B6, CYP2C9, and CYP3A4 genes served as candidate hub genes. AURKA, CDK1, CCNB1 and TOP2A were significantly upregulated and correlated with poor prognosis in hepatocellular carcinoma, AUC values of which were 95.7, 96.9, 98.1 and 96.1% respectively. There was not significant correlation between the expression of CYP2B6 and prognosis of hepatocellular carcinoma, while CYP2C9 and CYP3A4 genes were significantly downregulated and correlated with poor prognosis in hepatocellular carcinoma. AUC values of CYP2B6, CYP2C9, and CYP3A4 were 96.0, 97.0 and 88.0% respectively. In vitro, we further confirmed that curcumin significantly downregulated the expression of AURKA, CDK1, and TOP2A genes, while significantly upregulated the expression of CYP2B6, CYP2C9, and CYP3A4 genes.Our results provided a novel panel of AURKA, CDK1, TOP2A, CYP2C9, and CYP3A4 candidate genes for curcumin related chemotherapy of hepatocellular carcinoma.© 2022. The Author(s).

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出版当年[2021]版:
大类 | 3 区 医学
小类 | 2 区 全科医学与补充医学
最新[2025]版:
大类 | 3 区 医学
小类 | 2 区 全科医学与补充医学
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第一作者机构: [1]Scientifc Research Center, The First Afliated Hospital of Guangdong Pharmaceutical University, Gonghexiheng Street 1, Guangzhou, Guangdong 510080, P.R. China
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通讯作者:
通讯机构: [1]Scientifc Research Center, The First Afliated Hospital of Guangdong Pharmaceutical University, Gonghexiheng Street 1, Guangzhou, Guangdong 510080, P.R. China [3]Department of Traditional Chinese Medicine, The First Afliated Hospital of Guangdong Pharmaceutical University, Gonghexiheng Street 1, Guangzhou, Guangdong 510080, P.R. China. [4]Key Specialty of Clinical Pharmacy, The First Afliated Hospital of Guangdong Pharmaceutical University, Nonglin Down Street 19, Guangzhou, Guangdong 510080, P.R. China
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